Potential of SSR markers for plant breeding and variety identification in Australian barley germplasm
Potential of SSR markers for plant breeding and variety identification in Australian barley germplasm
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DOI：
10.1071/ar02178
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发表时间：
2003-01-01
影响因子：
--
通讯作者：
Langridge, P
中科院分区：
文献类型：
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作者：
Karakousis, A;Barr, AR;Langridge, P
与控制 16 个重要大麦性状的 18 个基因座紧密连锁的 SSR 标记在澳大利亚大麦育种计划中的适用性进行了评估。南澳大利亚大麦改良计划 (SABIP) 常规使用的一组 40 个基因型用于检查这些 SSR 标记对于标记辅助选择 (MAS) 的有用性。监测从供体系到受体系的性状位点的成功率在 10% 到 98% 之间，具体取决于标记。具有高多态信息含量 (PIC) 值的 SSR 被发现对于 MAS 中的应用最有用。评估还表明，源自基因组序列的 SSR 对于 MAS 比根据表达序列标签设计的 SSR 更成功。在 2 个澳大利亚大麦基因型组中筛选了总共 130 个 SSR 标记，以确定哪些标记对于区分澳大利亚种质最有用。通过该筛选产生的 PIC 值也与使用一组欧洲大麦基因型产生的值进行了比较。使用普通相关性（参数）、排名相关性（非参数）和部分相关性（多变量），在两个澳大利亚小组之间发现了很强的关联，但在两个澳大利亚小组和欧洲数据集之间没有观察到相关性或弱相关性。因此可以得出结论，用欧洲基因型筛选的 SSR 标记产生的 PIC 值不能用于预测 SSR 标记用于区分澳大利亚基因型的有用性。根据本研究生成的 PIC 值，选择了 36 个 SSR 标记来区分澳大利亚基因型。这些标记在澳大利亚和欧洲大麦基因型中均显示出高和/或一致的 PIC 值。
SSR markers closely linked to 18 loci that control 16 important barley traits were assessed for their applicability in Australian barley breeding programs. A panel of 40 genotypes routinely used by the South Australian Barley Improvement Program ( SABIP) was used to examine the usefulness of these SSR markers for marker assisted selection ( MAS). The success of monitoring a trait locus from donor to recipient lines ranged from 10 to 98%, depending on the marker. SSRs with a high polymorphic information content ( PIC) value were found to be the most useful for application in MAS. The assessment also indicated that SSRs derived from genomic sequences were more successful for MAS than those designed from expressed sequence tags. A total of 130 SSR markers were screened among 2 panels of Australian barley genotypes to determine which markers would be the most useful for discriminating Australian germplasm. PIC values generated by this screening were also compared with those generated using a panel of European barley genotypes. Using ordinary correlations ( parametric), rank correlations ( non- parametric), and partial correlations ( multi- variate), a strong association was found between the 2 Australian panels, but no or weak correlation was observed between the 2 Australian panels and the European dataset. It can therefore be concluded that PIC values generated by SSR markers screened with European genotypes cannot be used to predict the usefulness of an SSR marker for discriminating Australian genotypes. From PIC values generated in this study, 36 SSR markers have been selected for the discrimination of Australian genotypes. These markers all show high and/ or consistent PIC values among Australian and European barley genotypes.