[Clinical variations of chronic generalized periodontitis, genetic polymorphism and systemic production of inflammatory cytokines].
[Clinical variations of chronic generalized periodontitis, genetic polymorphism and systemic production of inflammatory cytokines].
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DOI：
10.17116/stomat201594511-16
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发表时间：
2015-01-01
期刊：
影响因子：
--
通讯作者：
Stepanov, S S
中科院分区：
文献类型：
--
作者：
Grigorovich, E Sh;Pomorgailo, E G;Stepanov, S S
细胞因子-白细胞介素IL-1 β、IL-1 RN、TNF α、IL-4基因多态性等位基因的携带可能是慢性牙周炎患者的特异性特征。基因检测可用于在疾病的早期表现中预测其病程。目的：目的：探讨牙周病临床表现、炎症细胞因子基因多态性与全身炎症细胞因子水平的关系。对150例牙周炎患者进行牙周组织评估和锥形束计算机断层扫描（CBCT）。对150例牙周炎患者和150例健康人进行了IL-1 β-511 C>T和+3953 C>T、IL-1 RN（VNTR内含子2）、IL-4（VNTR内含子3）、TNF α-308 G>A基因多态性等位基因的分子遗传学检测，并测定了外周血中IL-1 β、TNF α、IL-4的含量。根据牙周炎患者的临床表现和支持骨吸收的速度、性质，将患者分为“侵袭性”、“中度进展性”和“缓慢进展性”牙周炎病程组。疾病严重程度与多态性基因细胞因子IL-1 RN（VNTR内含子2）、TNF α-308 G>A和IL-4（VNTR内含子3）的基因型和等位基因分布相关;单倍型IL-1 β-511 TIL-1 β +3953 T/IL-1 RN 2 R。牙周炎组间IL-1 β、TNF α和IL-4的全身水平无统计学显著差异，但细胞因子的供体水平低2-4倍。
Carriage of polymorphic alleles of genes of cytokines-interleukines IL-1beta, IL-1RN, TNFalpha, IL-4 can be a specific feature of chronic periodontitis patients. Genetic tests can be used to predict the course of the disease at its early manifestations. Objective: To establish the relationship of clinical manifestations of periodontal disease, inflammatory cytokines gene polymorphism and systemic levels of cytokine production. Periodontal tissue assessment and cone-beam computed tomography (CBCT) were performed in 150 periodontitis patients. A molecular--genetic testing for the presence of polymorphic alleles of genes IL-1beta -511 C>T and +3953 C>T, IL-1RN (VNTR intron 2), IL-4 (VNTR intron 3), TNFalpha-308 G>A; content determined IL-1beta, TNFalpha, IL-4 in peripheral blood was carried out in 150 patients with periodontitis and 150 healthy donors. Based on the analysis of the speed and nature of the supporting bone resorption and clinical manifestations patients are divided in "aggressive", "moderately progressive" and "slowly progressive" periodontits course groups. Disease severity was associated with distribution of genotypes and alleles of polymorphic genes cytokine IL-1RN (VNTR intron 2), TNFalpha-308 G>A and IL-4 (VNTR intron 3); haplotype IL-1beta-511 TIL-1beta +3953 T/IL-1RN 2R. There was no statistically significant difference in systemic level of IL-1beta, TNFalpha and IL-4 between periodontitis groups but the donor level of cytokines was 2-4 times less.