Cell culture, oxidative stress, and antioxidants: avoiding pitfalls.
Cell culture, oxidative stress, and antioxidants: avoiding pitfalls.
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DOI：
10.4103/2319-4170.128725
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发表时间：
2014-05-01
影响因子：
5.5
通讯作者：
Halliwell, Barry
中科院分区：
文献类型：
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作者：
Halliwell, Barry
细胞培养被生物化学家和细胞/分子生物学家广泛使用，但培养中细胞暴露的O2水平波动（通常升高）会影响其许多特性。在某些细胞培养基中发现的低水平抗氧化剂也可以。添加到细胞培养基中的试剂，特别是“抗氧化剂”，可以与培养基的成分发生反应，产生H 2 O 2和降解产物，从而影响细胞行为。几篇发表的论文描述了抗坏血酸、多酚和类胡萝卜素的细胞效应，事实上，报道了由于这些“抗氧化剂”的降解产物的作用而产生的假象。“在自由基/抗氧化剂社区中，需要对细胞培养研究中的潜在人工制品有更高的认识。
Cell culture is widely used by biochemists and cell/molecular biologists, but the fluctuating (and often elevated) levels of O 2 to which cells in culture are exposed can affect many of their properties. So can the low level of antioxidants found in some cell culture media. Reagents, especially "antioxidants," added to cell culture media can react with the constituents of the media to produce H 2 O 2 and degradation products that can influence cell behavior. Several published papers describing the cellular effects of ascorbate, polyphenols, and carotenoids have, in fact, reported artifacts due to the actions of the degradation products of these "antioxidants." A greater awareness of the potential artifacts in cell culture studies is needed among the free radical/antioxidant community.