Differential proteomics: An overview of gel and non-gel based approaches
Differential proteomics: An overview of gel and non-gel based approaches
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DOI：
10.1093/bfgp/3.3.220
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发表时间：
2004-11-01
期刊：
影响因子：
--
通讯作者：
Albar, Juan Pablo
中科院分区：
文献类型：
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作者：
Monteoliva, Lucia;Albar, Juan Pablo
复杂生物系统中基因表达的综合分析需要开发新技术来研究细胞转录组和细胞蛋白质组。从概念和方法论的角度来看，每种方法都有优点和缺点。差异蛋白质组学，即不同蛋白质组（例如正常细胞与患病细胞、患病细胞与治疗细胞等）的比较至关重要。可以使用多种方法，这些方法通常涉及电泳和/或色谱法与化学或代谢标记和质谱法相结合。这些方法旨在识别参与不同病理生理状态的分子靶标，即蛋白质。将这些知识与其他技术的知识相结合，为分子水平的活性原理奠定了基础。在这里，将回顾在广泛的生物系统中用于研究差异表达蛋白质的各种基于凝胶和非凝胶的方法。
The comprehensive analysis of gene expression in complex biological systems has demanded the development of new technologies to study the cell transcriptome and the cell proteome. Each approach has advantages and disadvantages from both the conceptual and the methodological viewpoints. Differential proteomics, the comparison of distinct proteomes (eg normal versus diseased cells, diseased versus treated cells etc) is of paramount importance. Several approaches can be used and these typically involve electrophoresis and/or chromatography combined with chemical or metabolic labelling and mass spectrometry. These approaches aim to identify molecular targets, namely proteins, involved in different physiopathological states. Incorporating this knowledge with knowledge from other technologies lays the foundations of active principles at the molecular level. Here, the various gel- and non-gel-based approaches that are used in a wide range of biological systems for the study of differentially expressed proteins will be reviewed.