Sex-differential expression of metabolism-related genes in response to a high-fat diet
Sex-differential expression of metabolism-related genes in response to a high-fat diet
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DOI：
10.1038/oby.2007.117
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发表时间：
2008-04-01
期刊：
影响因子：
6.9
通讯作者：
Palou, Andreu
中科院分区：
文献类型：
--
作者：
Priego, Teresa;Sanchez, Juana;Palou, Andreu
目的：这项工作的目的是确定高脂肪饮食对大鼠脂质代谢和燃料分配相关基因表达的性别相关差异，以及这是否与雄性患代谢紊乱的更高倾向有关。方法和步骤：雄性和雌性Wistar大鼠分别饲喂正常脂肪（NF）或HF饮食6个月。测量体重、脂肪库重量、肝脏脂质浓度、血液代谢物以及肝脏、白色脂肪组织（WAT）和骨骼肌中参与燃料代谢和分配的基因表达。结果：在循环胰岛素水平不变的情况下，饲喂HF饮食的雌性大鼠体重增加更多，肥胖指数增加更大；这些动物还表现出与WAT能量流入和骨骼肌脂肪利用相关的基因表达增加。雄性大鼠肝脏过氧化物酶体增殖物激活受体α (ppar - α)和CPT1L mRNA表达增加，而雌性大鼠则没有，这表明该器官对脂质的处理和氧化增强，肝脏中甘油三酯含量较高。食用HF食物的雄性大鼠也表现出更高的血液胰岛素水平。讨论：这些结果表明，在HF饮食的反应中，脂肪处理和组织之间的分配存在性别依赖差异，女性在脂肪组织中储存脂肪的能力更高，在肌肉中氧化脂肪酸的能力更高。这些适应有助于解释在HF饮食条件下，女性患肥胖相关疾病的倾向较低。
objective: The aim of this work was to determine the sex-associated differences in the expression of genes related to lipid metabolism and fuel partitioning in response to a high-fat ( HF) diet in rats, and whether this is linked to the higher tendency of males to suffer from metabolic disorders.Methods and Procedures: Male and female Wistar rats were fed for 6 months on a normal-fat ( NF) or an HF diet. Body weight, fat depot weight, lipid concentration in liver, blood metabolites, and the expression of genes involved in fuel metabolism and partitioning in the liver, white adipose tissue ( WAT), and skeletal muscle were measured.Results: Female rats fed on an HF diet gained more weight and had a greater increase in the adiposity index than male rats, while the circulating insulin levels remained unaltered; these animals also showed an increased expression of genes related to the energy influx in WAT and with fat utilization in skeletal muscle. Male but not female rats showed increased hepatic peroxisome proliferator-activated receptor-alpha ( PPAR-alpha) and CPT1L mRNA expression, suggesting enhanced lipid handling and oxidation by this organ, and have a higher triacylglycerol content in liver. Male rats under the HF diet also displayed higher blood insulin levels.Discussion: These results show sex-dependent differences in lipid handling and partitioning between tissues in response to an HF diet, with females showing a higher capacity for storing fat in adipose tissue and for oxidizing fatty acids in muscle. These adaptations can help to explain the lower tendency of females to suffer from obesity-linked disorders under the conditions of an HF diet.