Identification and characteristics of insulin-like growth factor system in the brain, liver, and gonad during development of a seasonal breeding teleost, Pampus argenteus
Identification and characteristics of insulin-like growth factor system in the brain, liver, and gonad during development of a seasonal breeding teleost, Pampus argenteus
复制标题
DOI：
10.1016/j.ygcen.2020.113645
复制
发表时间：
2021-01-01
影响因子：
2.7
通讯作者：
Xu, Shanliang
中科院分区：
文献类型：
--
作者：
Gu, Weiwei;Yang, Yang;Xu, Shanliang
硬骨鱼类，特别是季节性繁殖的硬骨鱼类，其生殖活动与脑和肝的发育和功能密切相关。本研究测量了胰岛素样生长因子（IGF）系统参与控制繁殖的银鲳（Pampus argenteus），季节性繁殖的热带到温带的商业鱼类。我们克隆并鉴定了igfs（igf 2和igf 3）和igfrs（igf 1 ra，igf 1 rb和igf 2 r）的cDNA，并研究了它们的转录水平与季节性繁殖的关系。系统发育分析表明，银的2种IGF-1和IGF-2以及3种IGF-1 RA、IGF-1 RB和IGF-2 R与硬骨鱼类的IGF-3聚在一起，而IGF-3是一种跨膜蛋白，与其他硬骨鱼类的IGF-3不同。IGF-3在银鱼中的表达具有性腺特异性。igf 1在雌性大脑中的转录水平最高，而igfrs在两性大脑中的水平在配子发生过程中都有所增加。同时，在卵黄发生和精原细胞增殖过程中，igfs和igfrs在两性的肝脏和性腺中保持较高的转录水平。结论：脑、肝、性腺的发育和活动与IGF系统（IGFs和IGFRs）有关。IGFs主要表达于肝脏。然而，性腺发育，特别是卵黄发生和精原细胞增殖，与IGFs在该物种。
Reproductive activity is closely related to the development and function of the brain and liver in teleosts, particularly in seasonal breeding teleosts. This study measured the involvement of the insulin-like growth factor (IGF) system in controlling the reproduction of the silver pomfret Pampus argenteus, a seasonal breeding tropical to temperate commercial fish. We cloned and characterized the cDNAs of igfs (igf2 and igf3) and igfrs (igf1ra, igf1rb, and igf2r) and examined their transcript levels in relation to seasonal reproduction. Phylogenetic analyses revealed that two types of IGFs (IGF-1 and IGF-2) and three types of IGFRs (IGF1RA, IGF1RB, and IGF2R) of the silver pomfret were clustered with those of teleosts; however, IGF-3 was a transmembrane protein different with the IGF-3 of other teleosts. The expression of IGF-3 was gonad-specific in the silver pomfret. The transcript levels of igf1 in the female brain were the highest, and the levels of igfrs in both sexes' brains increased during gametogenesis. Meanwhile, igfs and igfrs maintained high transcript levels in both sexes' liver and gonad during vitellogenesis and spermatogonia proliferation. We concluded that the development and activities of brain, liver, and gonad were related to the IGF system (IGFs and IGFRs). And the IGFs were mainly expressed in the liver. Nevertheless, gonadal development, especially vitellogenesis and spermatogonia proliferation, were related with IGFs in this species.