Viability and growth of mouse embryos after in vitro culture and fusion.
Viability and growth of mouse embryos after in vitro culture and fusion.
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体外培养和融合后小鼠胚胎的活力和生长。
DOI：
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发表时间：
1970
期刊：
影响因子：
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通讯作者：
A. McLaren
中科院分区：
文献类型：
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作者：
P. Bowman;A. McLaren
约80%的8-细胞小鼠卵在培养中发育到胚泡阶段，无论透明质酸是保持完整，还是用链霉蛋白酶去除（预孵育和透析），然后将卵单独培养或作为融合对培养。当使用链霉蛋白酶而没有预先孵育和透析时，成功率降低到50%。在转移到子宫寄养母体后，20- 30%的在有或没有胚胎的情况下单独或融合培养的明显正常的胚泡发育成活胎，而直接从子宫取出的对照胚泡超过50%。一些培养胚胎的过度死亡发生在植入前，一些发生在植入后不久。来自培养囊胚的胎仔平均比来自类似转移的对照子宫囊胚的胎仔轻0·1 g。未观察到胎盘重量差异。胎儿和胎盘重量不受卵是否单独培养或融合的影响，这意味着融合胚胎的生长调节在妊娠第17天完成。卵在培养中保持的时间越长，它们在转移后的存活率就越低，由此产生的胎儿就越轻。
About 80 % of 8-cell mouse eggs developed to the blastocyst stage in culture, whether the zona pellucida was left intact, or removed with pronase (pre-incubated and dialysed) and the eggs then cultured singly or as fused pairs. When pronase was used without prior incubation and dialysis, the success rate was reduced to 50 %. After transfer to uterine foster-mothers, 20–30 % of apparently normal blastocysts cultured with or without the zona, singly or fused, developed into live foetuses, compared with over 50 % of control blastocysts taken directly from the uterus. Some of the excess mortality of cultured embryos took place before implantation and some soon after. The foetuses derived from cultured blastocysts averaged 0·1 g lighter than those derived from control uterine blastocysts similarly transferred. No differences in the weights of the placentae were observed. Foetal and placental weights were unaffected by whether the eggs had been cultured singly or fused, implying that growth regulation of fused embryos is complete by the 17th day of gestation. The longer the eggs were maintained in culture, the lower was their viability after transfer, and the lighter were the foetuses derived from them.