Monitoring monkeypox virus in saliva and air samples in Spain: a cross-sectional study.
Monitoring monkeypox virus in saliva and air samples in Spain: a cross-sectional study.
复制标题
DOI：
10.1016/s2666-5247(22)00291-9
复制
发表时间：
2023-01
期刊：
影响因子：
38.2
通讯作者：
Alcami, Antonio
中科院分区：
文献类型：
--
作者：
Hernaez, Bruno;Munoz-Gomez, Ana;Sanchiz, Africa;Orviz, Eva;Valls-Carbo, Adrian;Sagastagoitia, Inigo;Ayerdi, Oskar;Martin, Rocio;Puerta, Teresa;Vera, Mar;Cabello, Noemi;Vergas, Jorge;Prieto, Cristina;Pardo-Figuerez, Maria;Negredo, Anabel;Lagaron, Jose Maria;del Romero, Jorge;Estrada, Vicente;Alcami, Antonio
猴痘病毒通过直接接触传播，但通过唾液或呼出的飞沫和气溶胶传播尚未得到研究。我们的目的是评估感染猴痘病毒的患者的唾液样本、飞沫和气溶胶中是否存在猴痘病毒 DNA 和传染性病毒。我们对西班牙马德里两个卫生中心就诊的经 PCR 确诊的猴痘患者进行了一项横断面研究。对于每位患者，我们收集了唾液样本、面罩内呼出的液滴样本以及通过新开发的纳米纤维过滤器进行空气过滤捕获的气溶胶样本。我们通过定量 PCR (qPCR) 检测病毒 DNA 并在细胞培养物中分离感染性病毒，评估了样本中是否存在猴痘病毒。 2022年5月18日至7月15日期间，44名有症状的猴痘患者就诊于马德里的两个卫生中心，并被纳入该研究。所有患者均为顺性别男性，中位年龄为 35·0 岁 (IQR 11·3)。我们通过 qPCR 在 41 份唾液样本中的 35 份 (85%) 中发现了高含量的猴痘病毒 DNA。从 33 份猴痘病毒 DNA 呈阳性的唾液样本中的 22 份（67%）中回收到传染性猴痘病毒。我们还发现受影响的皮肤区域或一般症状的数量与唾液样本中存在的病毒载量之间存在显着关联。在口罩内检测到的猴痘患者呼出的飞沫中，45 个样本中的 32 个（71%）含有猴痘病毒 DNA，其中 32 个阳性样本中有两个显示存在传染性病毒。尽管患者在就诊期间佩戴了 FFP2 口罩，但在 42 个样本中的 27 个样本中检测到了从医疗咨询室收集的气溶胶中的猴痘病毒 DNA（64%）。没有从气溶胶样本中回收传染性病毒。从收容猴痘患者的医院隔离室收集的气溶胶中发现了高水平的猴痘病毒 DNA。在大多数猴痘患者的唾液中鉴定出高活猴痘病毒载量，以及在飞沫和气溶胶中发现猴痘病毒 DNA，需要进一步的流行病学研究来评估 2022 年猴痘病毒爆发中呼吸道感染途径的潜在相关性。欧盟、Consejo Superior de Investigaciones Científicas 和 Ciberinfec。
The transmission of monkeypox virus occurs through direct contact, but transmission through saliva or exhaled droplets and aerosols has not yet been investigated. We aimed to assess the presence of monkeypox virus DNA and infectious virus in saliva samples and droplets and aerosols exhaled from patients infected with monkeypox virus. We did a cross-sectional study in patients with monkeypox confirmed by PCR who attended two health centres in Madrid, Spain. For each patient, we collected samples of saliva, exhaled droplets within a mask, and aerosols captured by air filtration through newly developed nanofiber filters. We evaluated the presence of monkeypox virus in the samples by viral DNA detection by quantitative PCR (qPCR) and isolation of infectious viruses in cell cultures. Between May 18 and July 15, 2022, 44 patients with symptomatic monkeypox attended two health centres in Madrid and were included in the study. All were cisgender men, with a median age of 35·0 years (IQR 11·3). We identified high loads of monkeypox virus DNA by qPCR in 35 (85%) of 41 saliva samples. Infectious monkeypox virus was recovered from 22 (67%) of 33 saliva samples positive for monkeypox virus DNA. We also found a significant association between the number of affected cutaneous areas or general symptoms and the viral load present in saliva samples. Droplets exhaled from patients with monkeypox, detected inside a mask, contained monkeypox virus DNA in 32 (71%) of 45 samples, with two of the 32 positive samples showing the presence of the infectious virus. Monkeypox virus DNA in aerosols, collected from the medical consultation room, were detected in 27 (64%) of 42 samples, despite patients wearing an FFP2 mask during the visit. Infectious virus was not recovered from aerosol samples. High levels of monkeypox virus DNA were identified in aerosols collected from a hospital isolation room housing a patient with monkeypox. The identification of high viable monkeypox virus loads in saliva in most patients with monkeypox and the finding of monkeypox virus DNA in droplets and aerosols warrants further epidemiological studies to evaluate the potential relevance of the respiratory route of infection in the 2022 monkeypox virus outbreak. EU, Consejo Superior de Investigaciones Científicas, and Ciberinfec.