Refined mapping of allele loss at chromosome 10q23-26 in prostate cancer
Refined mapping of allele loss at chromosome 10q23-26 in prostate cancer
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DOI：
10.1002/pros.10038
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发表时间：
2002-02-15
期刊：
影响因子：
2.8
通讯作者：
Royer-Pokora, B
中科院分区：
文献类型：
--
作者：
Leube, B;Drechsler, M;Royer-Pokora, B
背景。在前列腺癌中描述了10q中至少两个片段的等位基因丢失，一个定位于PTEN基因，另一个定位于远端，在晚期前列腺癌中丢失更为频繁。利用微卫星标记密集图谱研究了59例前列腺癌样本中10q23 ~ q26之间63 cM区域的等位基因丢失（LOH）。在13/59的肿瘤中观察到10q中至少一个标记物的缺失。LOH随分级和分期增加。详细的缺失定位确定了三个等位基因缺失区域，第一个区域定位于PTEN基因的位置，第二个区域定位于一个肿瘤中一个标记D10S1692的缺失，第三个区域定位于标记D10S1757和D10S587之间，包括DMBT，标记D10S209和D10S1679之间约1.2 Mb的定位区域，在一个肿瘤中丢失。PTEN基因的LOH是常见的，但sscp筛选未检测到其余等位基因的突变。可能有两个以上的肿瘤抑制（TS）基因定位在PTEN的远端。这些假定的TS基因的位置现在可以用一组密集的精确定位标记在更大范围的晚期肿瘤中进行定位。中华医学会杂志，2002。(C) 2002 Wiley-Liss, Inc。
BACKGROUND. Allele loss of at least two segments in 10q, one mapping to the PTEN gene and one more distal were described in prostate cancer, with loss more frequent in advanced prostate cancer.METHODS. A 63 cM region from 10q23 to q26 was studied for allele loss (LOH) in 59 prostate cancer samples using a dense map of microsatellite markers.RESULTS. LOH of at least one marker in 10q was observed in 13/59 tumors. LOH increased with grade and stage. Detailed deletion mapping identified three regions of allele loss, The first region mapped to the site of the PTEN gene, the second is defined by loss of one marker, D10S1692, in one tumor, and the third is defined between markers D10S1757 and D10S587, including DMBT, with a subregion of approximately 1.2 Mb mapping between markers D10S209 and D10S1679, lost in one tumor.CONCLUSIONS. LOH at the PTEN gene is frequent but mutations in the remaining allele were not detected by SSCP-screening. There may be more than two tumor suppressor (TS) genes mapping more distal of PTEN. The site for these putative TS genes can now be mapped with a dense set of precisely localized markers in a larger series of advanced tumors. Prostate 50: 135-144, 2002. (C) 2002 Wiley-Liss, Inc.