Spherical carrier amplification strategy for electrochemical immunosensor based on polystyrene-gold nanorods @L-cysteine/MoS2 for determination of tacrolimus
Spherical carrier amplification strategy for electrochemical immunosensor based on polystyrene-gold nanorods @L-cysteine/MoS2 for determination of tacrolimus
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基于聚苯乙烯-金纳米棒@L-半胱氨酸/MoS2的电化学免疫传感器球形载体扩增策略测定他克莫司
DOI：
10.1016/j.talanta.2020.121321
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发表时间：
2020
期刊：
影响因子：
6.1
通讯作者：
Chang Dong
中科院分区：
文献类型：
--
作者：
Zhang Ze;Zhang Yingcong;Yu Hongwei;Rong Shengzhong;Gao Hongmin;Meng Lingqiang;Dai Jianmin;Pan Hongzhi;Chang Dong
基于球形载体扩增策略，设计了一种新型的测定他克莫司的电化学免疫传感器。在这项工作中，通过将金纳米棒（AuNRs）功能化的L-半胱氨酸（AuNRs@L-Cys）缀合到聚苯乙烯纳米颗粒（PS）上形成纳米结构来制备球形信号载体，大大增加了负载的捕获抗体的量。PS是一种稳定的球形功能聚合物，具有较大的比表面积，通过偶联剂将其标记为AuNRs@L-Cys，以增加活性基团、生物相容性和导电性。捕获抗体可以通过连接试剂戊二醛（GA）连接在PS-AuNR @L-Cys上。此外，利用壳聚糖固定的单层二硫化钼（MoS 2）构建了该免疫传感器的基底，提高了电极的承载能力和稳定性。电化学还原后，MoS 2的电导率和电子传递速率明显提高，为该免疫传感器提供了平台，进一步放大了信号。在优化的条件下，所提出的免疫传感器显示了线性的Tac浓度行为从1.0到30 ng mL− 1，检测限为0.17 ng mL−1（S/N = 3）。该方法成功地应用于血清样品中Tac的定量测定，具有可接受的精密度。结果表明，一个潜在的分析平台，可用于识别其他药物的浓度。
A novel sensitive electrochemical immunosensor has been designed for determination of tacrolimus (Tac) based on spherical carrier amplification strategy. In this work, a spherical signal carrier was prepared by conjugating gold nanorods (AuNRs) functionalized L-cysteine (AuNRs@L-Cys) onto polystyrene nanoparticles (PS) to form a nanostructure, greatly increasing the amount of loaded capture antibodies. The PS was a stable spherical functional polymer with large specific surface area and was labeled with AuNRs@L-Cys via coupling reagent to increase active groups, biocompatibility and conductivity. The capture antibodies could be attached on the PS- AuNRs@L-Cys via linkage reagent glutaraldehyde (GA). Furthermore, single-layer molybdenum disulfide (MoS2) fixed by chitosan were utilized to construct the base of this immunosensor, increasing the carrying capacity and stability of the electrode. After electrochemical reduction, the conductivity and electron transfer rate of MoS2were obviously improved, which offered a platform for this immunosensor and further amplified the signal. Under the optimized conditions, the proposed immunosensor revealed a linear Tac-concentration behavior from 1.0 to 30 ng mL−1with a detection limit of 0.17 ng mL−1(S/N = 3). The immunoassay was successfully applied to the quantification of Tac in serum samples with acceptable precision. The results indicated that a potentially analytical platform may be used to recognize the concentration of other drugs.