Development of introgression lines of an Indica-type rice variety, IR64, for unique agronomic traits and detection of the responsible chromosomal regions
Development of introgression lines of an Indica-type rice variety, IR64, for unique agronomic traits and detection of the responsible chromosomal regions
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DOI：
10.1016/j.fcr.2009.08.004
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发表时间：
2009-11-10
影响因子：
5.8
通讯作者：
Kobayashi, Nobuya
中科院分区：
文献类型：
--
作者：
Fujita, Daisuke;Santos, Rizza E.;Kobayashi, Nobuya
共334个导入系（国际零部件进口限额：BC 3衍生系），其衍生自籼稻栽培品种IR 64的轮回亲本与10个供体亲本之间的杂交，包括新植物类型（NPT）系IR 65600 -87-2-2-3、IR 65598 -112-2、IR 65564 -2-2-3、IR 69093 -41-2-3-2、IR 69125 -25-3-1-1、已开发出Hoshiaoba、IR 66215 -44-2-3、IR 68522 -10-2-2、IR 71195-AC 1和IR 66750 -6-2-1。2005 - 2007年，在国际水稻研究所对这些具有IR 64遗传背景的INLs进行了8个农艺性状的表征：抽穗天数、秆长、叶宽、叶长、穗长、穗数、百粒重和每穗总小穗数。为了鉴定来自供体亲本的渐渗片段，使用200多个多态性简单重复序列标记检测334个INLs的基因型。在第1、2、4、5和6号染色体上检测到的这些片段通常在来自四个以上供体品种的INLs中渗入。根据334个INLs的表型和基因型数据，分析了334个INLs的农艺性状与渗入染色体片段的相关性。8个性状共检测到54个区域：齐穗天数7个区域、秆长8个区域、叶宽8个区域、叶长4个区域、穗长6个区域、单株穗数3个区域、百粒重7个区域、每穗总小穗数11个区域。其中，第4染色体长臂上的区域与NPT的长叶、宽叶、高小穗数等性状相关。所开发的334个具有IR 64遗传背景的INLs将为农艺性状的遗传分析提供有用的材料。(C)2009爱思唯尔有限公司版权所有。
A total of 334 introgression lines (INLs: BC3-derived lines) derived from crosses between a recurrent parent of Indica rice cultivar IR64 and 10 donor parents, including new plant type (NPT) lines IR65600-87-2-2-3, IR65598-112-2, IR65564-2-2-3, IR69093-41-2-3-2, IR69125-25-3-1-1, Hoshiaoba, IR66215-44-2-3, IR68522-10-2-2, IR71195-AC1, and IR66750-6-2-1, have been developed. These INLs with IR64 genetic background were characterized for eight agronomic traits: days to heading, culm length, leaf width, leaf length, panicle length, panicle number, 100-grain weight, and total spikelet number per panicle at the International Rice Research Institute from 2005 to 2007. To identify introgressed segments from the donor parents, genotypes of the 334 INLs were detected using more than 200 polymorphic simple sequence repeat markers. These segments detected on chromosomes 1, 2, 4, 5, and 6 were commonly introgressed across the INLs from more than four donor varieties. Based on the data of phenotype and genotype for the 334 INLs, associations between agronomic traits and introgressed chromosomal segments in the 334 INLs were investigated. A total of 54 regions for the eight traits were detected: seven regions for days to heading, eight regions for culm length, eight regions for leaf width, four regions for leaf length, six regions for panicle length, three regions for panicle number per plant, seven regions for 100-grain weight, and 11 regions for total spikelet number per panicle. Among them, the region on the long arm of chromosome 4 was associated with characteristics of the NPT such as long leaf, broad leaf, and high spikelet number. The developed 334 INLs with the IR64 genetic background will be useful materials for genetic analysis of agronomic traits. (C) 2009 Elsevier B.V. All rights reserved.