Evolution of Wolbachia-induced cytoplasmic incompatibility in Drosophila simulans and D-sechellia
Evolution of Wolbachia-induced cytoplasmic incompatibility in Drosophila simulans and D-sechellia
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DOI：
10.1111/j.0014-3820.2002.tb00187.x
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发表时间：
2002-09-01
期刊：
影响因子：
3.3
通讯作者：
Merçot, H
中科院分区：
文献类型：
--
作者：
Charlat, S;Nirgianaki, A;Merçot, H
胞内细菌沃尔巴克氏体通过各种机制侵入节肢动物宿主种群，其中最常见的是细胞质不相容性（Q。当感染的雄性与未感染的雌性交配或雌性感染不同的、不相容的沃尔巴克氏体菌株时，Cl涉及胚胎死亡率升高。本研究的重点是两种果蝇的这种现象：D。simulans和D. sechellia。模拟果蝇种群被几种沃尔巴克氏体菌株感染，包括wHa和wNo。sechellia果蝇只被两种Wolbachia感染：wSh和wSn。在两种果蝇中均发现了Wolbachia的双重感染，几个分子标记表明wHa与wSh密切相关，wNo与wSn密切相关。此外，两种宿主的双重感染与密切相关的线粒体单倍型有关，即siI（与D. simulans）和se（与D. sechellia）。为了验证沃尔巴克氏体相容性类型可以迅速分化的理论预测，我们将wSh和wSn注入D。simulans，比较它们的CI特性，它们的姐妹株wHa和wNo，分别在相同的宿主遗传背景。我们发现，在每对姐妹株Cl水平是相似的，姐妹株是完全兼容的。我们的结论是，沃尔巴克氏体姐妹株已经发展分离彼此的短时期是不够的，他们的Cl属性显着分歧。
The intracellular bacterium Wolbachia invades arthropod host populations through various mechanisms, the most common of which being cytoplasmic incompatibility (Q. Cl involves elevated embryo mortality when infected males mate with uninfected females or females infected with different, incompatible Wolbachia strains. The present study focuses on this phenomenon in two Drosophila species: D. simulans and D. sechellia. Drosophila simulans populations are infected by several Wolbachia strains, including wHa and wNo. Drosophila sechellia is infected by only two Wolbachia: wSh and wSn. In both Drosophila species, double infections with Wolbachia are found. As indicated by several molecular markers, wHa is closely related to wSh, and wNo to wSn. Furthermore, the double infections in the two host species are associated with closely related mitochondrial haplotypes, namely siI (associated with wHa and wNo in D. simulans) and se (associated with wSh and wSn in D. sechellia). To test the theoretical prediction that Wolbachia compatibility types can diverge rapidly, we injected wSh and wSn into D. simulans, to compare their CI properties to those of their sister strains wHa and wNo, respectively, in the same host genetic background. We found that within each pair of sister strains Cl levels were similar and that sister strains were fully compatible. We conclude that the short period for which the Wolbachia sister strains have been evolving separated from each other was not sufficient for their Cl properties to diverge significantly.