Abolition of Biofilm Formation in Urinary Tract Escherichia coli and Klebsiella Isolates by Metal Interference through Competition for Fur
Abolition of Biofilm Formation in Urinary Tract Escherichia coli and Klebsiella Isolates by Metal Interference through Competition for Fur
复制标题
DOI：
10.1128/aem.00241-10
复制
发表时间：
2010-06-01
影响因子：
4.4
通讯作者：
Klemm, Per
中科院分区：
文献类型：
--
作者：
Hancock, Viktoria;Dahl, Malin;Klemm, Per
细菌生物膜与大量的持续性和慢性感染有关。居住在生物膜上的细菌对抗生素和免疫防御特别有抵抗力，这使得根除与生物膜相关的感染即使不是不可能，也很难。在尿道中，游离铁是严格限制的，但对细菌生长至关重要。与生物膜相关的大肠杆菌细胞尤其需要铁。抑制生物膜形成的一个有吸引力的方法是欺骗细菌的铁摄取调节系统。在这里，我们证明了添加二价金属离子（如Zn（II）和Co(II)）可以破坏生物膜的形成，这是因为它们对铁摄取的主要控制蛋白Fur具有高于铁的亲和力，从而抑制铁的摄取。在微量滴度板和流室以及导尿管上观察到锌（II）存在时尿路感染性大肠杆菌菌株的生物膜形成减少。这些结果进一步支持铁摄取对生物膜的形成确实至关重要，因此，针对这些摄取系统可能是消除感染菌株引起的生物膜的有效方法。
Bacterial biofilms are associated with a large number of persistent and chronic infections. Biofilm-dwelling bacteria are particularly resistant to antibiotics and immune defenses, which makes it hard if not impossible to eradicate biofilm-associated infections. In the urinary tract, free iron is strictly limited but is critical for bacterial growth. Biofilm-associated Escherichia coli cells are particularly desperate for iron. An attractive way of inhibiting biofilm formation is to fool the bacterial regulatory system for iron uptake. Here, we demonstrate that biofilm formation can be impaired by the addition of divalent metal ions, such as Zn(II) and Co(II), which inhibit iron uptake by virtue of their higher-than-iron affinity for the master controller protein of iron uptake, Fur. Reduced biofilm formation of urinary tract-infectious E. coli strains in the presence of Zn(II) was observed in microtiter plates and flow chambers as well as on urinary catheters. These results further support that iron uptake is indeed crucial for biofilm formation, and thereby, targeting these uptake systems might be an effective way to eradicate biofilms caused by infectious strains.