Quinine and citric acid elicit distinctive Fos-like immunoreactivity in the rat nucleus of the solitary tract
Quinine and citric acid elicit distinctive Fos-like immunoreactivity in the rat nucleus of the solitary tract
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DOI：
10.1152/ajpregu.00590.2001
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发表时间：
2002-06-01
影响因子：
2.8
通讯作者：
Travers, SP
中科院分区：
文献类型：
--
作者：
Travers, SP
本实验观察了清醒行为大鼠口服0.1M柠檬酸、0.3M氯化钠和0.3-30 mM奎宁(QHCl)后，孤束核(NST)内Fos样免疫反应(Fos)的变化。随着QHCl浓度的增加，中间体(I(R))和吻部(R)NST的FLI标记神经元数量增加，但FLI的局部分布在QHCl浓度之间是一致的，与柠檬酸的影响相比是不同的。奎宁引起的FLI集中在核的内侧1/3，酸性引起的FLI分布更广，集中在更远的外侧。令人惊讶的是，与QHCl和柠檬酸相反，氯化钠产生的FLI与水产生的FLI几乎没有区别。虽然这些模式的功能意义尚不清楚，但柠檬酸和QHCl是非首选刺激，但产生了不同的运动行为。QHCl(30 MM)可引起3.2倍于柠檬酸(0.1M)的张口，而酸可引起更多的摄取反应。Fli表达的平行差异表明，不同的NST区域可能在触发口腔运动行为方面具有不同的作用。
The present experiment investigated Fos-like immunoreactivity (FLI) in the nucleus of the solitary tract (NST) after intraoral infusions of 0.1 M citric acid, 0.3 M NaCl, and 0.3-30 mM quinine monohydrochloride (QHCl) in awake, behaving rats. Increases in QHCl concentration produced increases in the numbers of FLI-labeled neurons in the rostral part of the intermediate (i(r)) and rostral (r) NST, but the topographic distribution of FLI was consistent across QHCl concentrations and distinctive compared with effects of citric acid. Quinine elicited FLI concentrated in the medial third of the nucleus; acid elicited more broadly distributed FLI concentrated farther laterally. Surprisingly, in contrast to QHCl and citric acid, NaCl produced FLI that was indistinguishable from that produced by water. Although the functional significance of these patterns is unknown, citric acid and QHCl are nonpreferred stimuli but produced different oromotor behaviors. QHCl (30 mM) elicited similar to3.2 times as many gapes as citric acid (0.1 M), and acid elicited more ingestive responses. Parallel differences in FLI expression suggest that different NST regions may have distinctive roles in triggering oromotor behaviors.