Microwave fixation and localization of calcium in synaptic terminals using x-ray microanalysis and electron energy loss spectroscopy imaging
Microwave fixation and localization of calcium in synaptic terminals using x-ray microanalysis and electron energy loss spectroscopy imaging
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DOI：
10.1016/s0361-9230(96)00428-5
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发表时间：
1997-01-01
影响因子：
3.8
通讯作者：
Hasegawa, H
中科院分区：
文献类型：
--
作者：
Mizuhira, V;Hasegawa, H
钙离子在突触末梢的分布是通过大鼠脑中钙离子的两步化学沉淀来证明的。采用草酸钾/锑酸钾化学置换法，同时进行计算机微波辐射。通过计算机电子探针X射线显微分析（EDX）和电子能量损失光谱（EELS）成像研究了神经细胞结构中的这种沉淀。EDX测定值与标准样品和锑酸钙的理论值一致。典型的EELS光谱的Ca：L，O：K，和Sb：M从神经末梢在相同的组织块，用于EDX分析。在减去它们的背景图像之后，获得了良好的净Ca：L和Sb：M EELS数字图像。钙离子分布于神经末梢、突触囊泡、线粒体和突触膜。(C)1997年爱思唯尔科学公司
The distribution of calcium ions is demonstrated in synaptic terminals by means of a two-step chemical precipitation of calcium ions in the rat brain. K-oxalate/K-antimonate chemical replacement with simultaneous computerized microwave irradiation was used. This precipitate in nerve cell structures was investigated by computerized electron probe x-ray microanalysis (EDX) and electron energy loss spectroscopic (EELS) imaging. The values obtained by EDX agreed with those of the standard sample and theoretical values of Ca-antimonate. Typical EELS spectra of Ca:L, O:K, and Sb:M were obtained from nerve terminals in the same tissue block as that used for EDX analysis. Excellent net Ca:L and Sb:M EELS digital images were obtained after their background images were subtracted. Calcium ions were distributed in the nerve terminals, synaptic vesicles, mitochondria, and synaptic membranes. (C) 1997 Elsevier Science Inc.