Radioimmunoassay of Estradiol-17β Without Chromatography
Radioimmunoassay of Estradiol-17β Without Chromatography
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不用色谱法测定雌二醇-17β的放射免疫分析
DOI：
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发表时间：
1974
期刊：
影响因子：
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通讯作者：
A. Midgley
中科院分区：
文献类型：
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作者：
B. England;G. Niswender;A. Midgley
本文报道了几种测定血清雌二醇-17β的放射免疫分析方法。为了产生抗血清，这些方法中的大多数都使用了通过A或D环上的位点与载体蛋白结合的类固醇。由此产生的抗血清与相关类固醇表现出相当大的交叉反应，因此在实际的放射免疫分析之前需要使用各种层析分离步骤。半抗原的使用使载体蛋白通过B环(1，2，3)上的碳6连接到类固醇分子上，从而产生了高度特异的抗血清。蛋白质分子与其他位置的结合，使A环和D环可供抗体识别，也将导致产生具有增强特异性的抗体。本文报道了11β-17-雌二醇-17-β半琥珀酸衍生物与牛血清白蛋白偶联的抗血清。所制备的抗血清对17种β-雌激素类药物具有特异性。
ABSTRACT Several radioimmunoassays for the estimation of estradiol-17β in serum have been reported. For the generation of antisera, the majority of these methods have used steroids conjugated to a carrier protein through sites on the A or D rings. The resulting antisera have shown considerable cross reactivity with related steroids necessitating the use of various chromatographic separation steps prior to the actual radioimmunoassay. The use of haptens in which the carrier protein was conjugated to a steroid molecule through carbon 6 on the B ring (1,2,3) has resulted in the production of highly specific antisera. Conjugation of the protein molecule to other positions which leave the A and D rings available for recognition by antibody should also result in the generation of antibodies with enhanced specificity. In this report, an antiserum was prepared to a conjugate of an 11β-hemisuccinate derivative of estradiol-17β to bovine serum albumin. The resulting antiserum appeared to be specific for 17β-estradi...