Ginkgolide A Ameliorates LPS-Induced Inflammatory Responses In Vitro and In Vivo.
Ginkgolide A Ameliorates LPS-Induced Inflammatory Responses In Vitro and In Vivo.
复制标题
银杏内酯 A 可改善 LPS 引起的体外和体内炎症反应。
DOI：
10.3390/ijms18040794
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发表时间：
2017-04-10
影响因子：
5.6
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Li Y;Wu Y;Yao X;Hao F;Yu C;Bao Y;Wu Y;Song Z;Sun Y;Zheng L;Wang G;Huang Y;Sun L;Li Y
Ginkgolide A (GA) is a natural compound isolated from Ginkgo biloba and has been used to treat cardiovascular diseases and diabetic vascular complications. However, only a few studies have been conducted on the anti-inflammatory effects of GA. In particular, no related reports have been published in a common inflammation model of lipopolysaccharide (LPS)-stimulated macrophages, and the anti-inflammatory mechanisms of GA have not been fully elucidated. In the present study, we extensively investigated the anti-inflammatory potential of GA in vitro and in vivo. We showed that GA could suppress the expression of pro-inflammatory mediators (cyclooxygenase-2 (COX-2) and nitric oxide (NO) and pro-inflammatory cytokines (tumor necrosis factor (TNF)-α, interleukin (IL)-6 and IL-1β) in LPS-treated mouse peritoneal macrophages, mouse macrophage RAW264.7 cells, and differentiated human monocytes (dTHP-1) in vitro. These effects were partially carried out via downregulating Nuclear factor kappa-B (NF-κB), Mitogen-activated protein kinases (MAPKs) (p38 mitogen-activated protein kinase and extracellular signal-regulated kinase (ERK), but not c-Jun N-terminal kinase (JNK), and activating the AMP-activated protein kinase (AMPK) signaling pathway also seems to be important. Consistently, GA was also shown to inhibit the LPS-stimulated release of TNF-α and IL-6 in mice. Taken together, these findings suggest that GA can serve as an effective inflammatory inhibitor in vitro and in vivo.
