Genes involved in auxin biosynthesis, transport and signalling underlie the extreme adventitious root phenotype of the tomato aer mutant
Genes involved in auxin biosynthesis, transport and signalling underlie the extreme adventitious root phenotype of the tomato aer mutant
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DOI：
10.1007/s00122-024-04570-8
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发表时间：
2024-04-01
影响因子：
5.4
通讯作者：
Thompson，Andrew J.
中科院分区：
文献类型：
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作者：
Kevei，Zoltan;Larriba，Eduardo;Thompson，Andrew J.
番茄砧木的使用有助于缓解气候变化不利影响引发的非生物压力飙升。侧根和不定根可以促进表层土壤的开发和养分吸收、地上部生物量和由此产生的总产量。了解根结构发育的遗传基础以及侧根和不定根是如何产生的是至关重要的。现有的具有特定根表型的突变系是分析和理解根发育性状的分子基础的极好资源。番茄气生根(Aer)突变体在初生茎上表现出极端的不定根表型。众所周知，这种表型与生长素从幼体向成熟茎的极地运输受限有关，但在本研究之前，导致这种表型的遗传位点尚不清楚。我们利用基因组学方法确定了气孔表型的多基因性质，并提供了证据表明，不同位置特定生长素生物合成、运输和信号基因的表达增加导致了番茄茎中不定根原基的形成。我们的结果允许使用分子标记选择不同水平的不定根，这可能有助于嫁接蔬菜作物的砧木育种策略，特别是在番茄中。在作为插穗进行营养繁殖的作物中，如果树和甘蔗果实，同源基因可能有助于选择更适合繁殖的品种。
The use of tomato rootstocks has helped to alleviate the soaring abiotic stresses provoked by the adverse effects of climate change. Lateral and adventitious roots can improve topsoil exploration and nutrient uptake, shoot biomass and resulting overall yield. It is essential to understand the genetic basis of root structure development and how lateral and adventitious roots are produced. Existing mutant lines with specific root phenotypes are an excellent resource to analyse and comprehend the molecular basis of root developmental traits. The tomatoaerial roots(aer) mutant exhibits an extreme adventitious rooting phenotype on the primary stem. It is known that this phenotype is associated with restricted polar auxin transport from the juvenile to the more mature stem, but prior to this study, the genetic loci responsible for theaerphenotype were unknown. We used genomic approaches to define the polygenic nature of theaerphenotype and provide evidence that increased expression of specific auxin biosynthesis, transport and signalling genes in different loci causes the initiation of adventitious root primordia in tomato stems. Our results allow the selection of different levels of adventitious rooting using molecular markers, potentially contributing to rootstock breeding strategies in grafted vegetable crops, especially in tomato. In crops vegetatively propagated as cuttings, such as fruit trees and cane fruits, orthologous genes may be useful for the selection of cultivars more amenable to propagation.