Mutational analysis of the human immunodeficiency virus: the orf-B region down-regulates virus replication.
Mutational analysis of the human immunodeficiency virus: the orf-B region down-regulates virus replication.
复制标题
人类免疫缺陷病毒突变分析：orf-B区下调病毒复制。
DOI：
10.1073/pnas.84.5.1434
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发表时间：
1987
影响因子：
11.1
通讯作者：
Levy,JA
中科院分区：
文献类型：
--
作者：
Luciw,PA;Cheng-Mayer,C;Levy,JA
通过重组DNA技术在人免疫缺陷病毒San弗朗西斯科分离株2（HIVSF 2）[以前是获得性免疫缺陷综合征相关逆转录病毒（ARV-2）的原型分离株]的感染性分子克隆中进行突变。这些变化对病毒复制和细胞病理学特性的影响通过将修饰的病毒克隆转染到培养的人细胞中来研究。gag、pol、env和达特区域的突变阻止了病毒在淋巴细胞中的复制和细胞病理学。orf-A的突变大大减少了病毒的复制，但并没有消除病毒的复制。orf-B区缺失的突变病毒具有高度致细胞病变性，复制水平比野生型病毒高约5倍。它们还在感染的淋巴细胞中产生比野生型病毒多约5倍的病毒DNA。因此，orf-B区域可起到下调病毒复制的作用。HIVSF 2基因组的突变分析是评估调节病毒复制和细胞病理学的基因的一种手段。
Mutations were made by recombinant DNA techniques in an infectious molecular clone of the human immunodeficiency virus San Francisco isolate 2 (HIVSF2) [formerly the prototype isolate of the acquired immunodeficiency syndrome-associated retrovirus (ARV-2)]. The effect of these changes on the replicative and cytopathologic properties of the virus was studied by transfecting modified virus clones into cultured human cells. Mutations in the gag, pol, env, and tat regions precluded virus replication and cytopathology in lymphoid cells. A mutation in orf-A dramatically reduced but did not abolish virus replication. Mutant viruses with deletions in the orf-B region were highly cytopathic and replicated to approximately 5-fold higher levels than wild-type virus. They also produced approximately 5-fold more viral DNA in infected lymphoid cells than did wild-type virus. Thus, the orf-B region may function to down-regulate virus replication. This mutational analysis of the HIVSF2 genome is a means of assessing genes regulating viral replication and cytopathology.