One-Step Cloning and Chromosomal Integration of DNA
One-Step Cloning and Chromosomal Integration of DNA
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DOI：
10.1021/sb400021j
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发表时间：
2013-09-01
影响因子：
4.7
通讯作者：
Shearwin, Keith E.
中科院分区：
文献类型：
--
作者：
St-Pierre, Francois;Cui, Lun;Shearwin, Keith E.
我们描述了克隆整合，一种将DNA整合到原核染色体中的方法，该方法在染色体外载体中克隆DNA的简单性。与现有技术相比，克隆整合大大减少了整合单个或多个DNA片段所需的时间和精力。此外，非整合促进了在典型的多拷贝质粒中不可能繁殖的遗传元件的克隆和表达。
We describe clonetegration, a method for integrating DNA into prokaryotic chromosomes that 'approaches the simplicity of cloning DNA within extrachromosomal vectors. Compared to existing techniques, clonetegration drastically decreases the time and effort needed for integration of singles or multiple DNA fragments. Additionally, donetegration facilitates cloning and expression of genetic elements that are impossible to propagate within typical multicopy plasmids.