Extracellular S-nitrosoglutathione, but not S-nitrosocysteine or N(2)O(3), mediates protein S-nitrosation in rat spinal cord slices.
Extracellular S-nitrosoglutathione, but not S-nitrosocysteine or N(2)O(3), mediates protein S-nitrosation in rat spinal cord slices.
复制标题
细胞外 S-亚硝基谷胱甘肽（而非 S-亚硝基半胱氨酸或 N(2)O(3)）介导大鼠脊髓切片中的蛋白质 S-亚硝化。
DOI：
10.1111/j.1471-4159.2006.04180.x
复制
发表时间：
2006
影响因子：
4.7
通讯作者：
Bizzozero,OscarA
中科院分区：
文献类型：
--
作者：
Romero,JorgeM;Bizzozero,OscarA
There is evidence that protein S‐nitrosothiols (PrSNOs) accumulate in inflammatory demyelinating disorders like multiple sclerosis and experimental allergic encephalomyelitis. However, very little is known regarding the mechanism by which PrSNOs are formed in target cells. The present study compares the ability of potential intercellular mediators of nitrosative damage including S‐nitrosoglutathione (GSNO), S‐nitrosocysteine and N2O3to induce protein S‐nitros(yl)ation in the spinal cord, a CNS region that is commonly affected in multiple sclerosis and experimental allergic encephalomyelitis. The results clearly demonstrate that while all three NO‐donors cause S‐nitrosation of proteins in cell‐free systems, only GSNO is a viable S‐nitrosating agent in rat spinal cord slices. Generation of PrSNOs with GSNO occurs by S‐transnitrosation as the process was not inhibited by either the NO‐scavenger rutin or the N2O3‐scavenger azide. Contrary to other cell types, nerve cells incorporate intact GSNO and neither functionall‐amino acid transporters nor cell‐surface thiols are required. We also found that there is a restricted number of proteins available for S‐nitrosation, even at high, non‐physiological concentrations of GSNO. These proteins are highly concentrated in mitochondria and mitochondria‐rich subcellular compartments. This study is relevant to those CNS disorders characterized by excessive nitric oxide production.
登录
查看更多内容
cAMP 依赖性蛋白激酶调节亚基的 cAMP 结合域与分解代谢基因激活蛋白是同源的。
DOI：
10.1073/pnas.79.24.7679
发表时间：
1982
影响因子：
11.1
作者：
I. Weber;K. Takio;K. Titani;T. Steitz
通讯作者：
T. Steitz
大鼠脂肪组织 cAMP 依赖性蛋白激酶：II 型的独特形式
影响因子：
4.1
作者：
S. Beebe;J. Corbin
通讯作者：
J. Corbin
DOI：
10.1073/pnas.83.18.6682
发表时间：
1986-09-01
影响因子：
11.1
作者：
MONTMINY, MR;SEVARINO, KA;GOODMAN, RH
通讯作者：
GOODMAN, RH
影响因子：
4.8
作者：
R. Jungmann;D. Kelley;M. Miles;D. Milkowski
通讯作者：
D. Milkowski
Swiss 3T3 细胞中三种细胞内信使系统（蛋白激酶 C、钙离子和环 AMP）参与 c-fos 基因表达的调节
影响因子：
3.5
作者：
T. Tsuda;Y. Hamamori;Takayuki Yamashita;Y. Fukumoto;Y. Takai
通讯作者：
Y. Takai