Effect of algicidal compound Nω-acetylhistamine on physiological response and algal toxins in Heterosigma akashiwo
Effect of algicidal compound Nω-acetylhistamine on physiological response and algal toxins in Heterosigma akashiwo
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杀藻化合物N-乙酰组胺对赤潮异藻生理反应及藻毒素的影响
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发表时间：
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影响因子：
6.8
通讯作者：
Ling Zhao
中科院分区：
文献类型：
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作者：
Qiuyin Zhu;Ling Zhao
赤潮异弯藻（Heterosigma akashiwo，Raphidophyceae）是一种有毒藻类，它能形成有害藻华（HABs），对水生生态系统和人类生活造成严重的负面影响。Nω-乙酰组胺（N-AcH）是溶藻细菌芽孢杆菌B1菌株分泌的一种溶藻化合物，它能抑制H.明石。本文对N-乙酰胆碱对H.探讨了赤霉素对小鼠的毒性变化。研究了用N-AcH处理的赤藻。用光密度法计算抑藻率，结果表明，对H。在40 μg/mLN-AcH培养基中处理96 h，赤霉素的转化率约为90%。经72 h处理后，透射电子显微镜（TEM）观察到H. Akashiwo细胞在该浓度下严重受损。叶绿素a和叶绿素B含量降低，丙二醛含量升高，超氧化物歧化酶活性和可溶性蛋白含量先升高后降低。GC-MS分析表明，处理48 h和96 h后，脂肪酸的种类和含量减少。溶血试验、MTT试验和微核试验均表明H.用40 μg/mL .N-AcH.总之，N-AcH主要杀死H。通过氧化应激作用对赤潮藻细胞产生抑制作用，并能降低其毒性，是一种具有杀藻和抑藻双重功能的有前途的杀藻剂。
The toxic alga Heterosigma akashiwo (Raphidophyceae) is known to form harmful algal blooms (HABs), which can .have serious negative effects on the aquatic ecosystem and human life. Previous study has shown that Nω- .acetylhistamine (N-AcH), an algicidal compound secreted by algicidal bacteria Bacillus sp. Strain B1, can inhibit .the growth of H. akashiwo. In this study, the algicidal mechanism of N-AcH against H. akashiwo was explored, and .the changes of toxicity of H. akashiwo treated with N-AcH were investigated. The algal inhibition rate was .calculated by the optical density method, and the results showed that the growth inhibition rate of H. akashiwo .was about 90% when treated in the medium with 40 μg/mL N-AcH at 96 h. After 72 h treatment, transmission .electron microscopy (TEM) showed that the microstructure of H. akashiwo cell was seriously damaged at this .concentration. The content of Chlorophyll a and Chlorophyll b decreased while malonaldehyde levels increased, .and superoxide dismutase activity first increased and then decreased as well as soluble protein content. GC-MS .revealed that the type and content of fatty acids cut down after 48 h and 96 h treatment. Hemolytic test, MTT .assay, and micronucleus test all demonstrated the decrease in the toxicity of H. akashiwo treated with 40 μg/mL .N-AcH. In brief, N-AcH mainly kills H. akashiwo cell through oxidative stress and can also reduce its toxicity, so it .is a promising algicide with the dual functions of killing algae and inhibiting algal toxic effects.