Antiprotozoal, Antimycobacterial and Cytotoxic Potential of Some British Green Algae
Antiprotozoal, Antimycobacterial and Cytotoxic Potential of Some British Green Algae
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DOI：
10.1002/ptr.3072
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发表时间：
2010-07-01
影响因子：
7.2
通讯作者：
Tasdemir, Deniz
中科院分区：
文献类型：
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作者：
Spavieri, Jasmine;Kaiser, Marcel;Tasdemir, Deniz
在继续寻找抗原生动物和抗结核分子的天然来源的过程中，我们筛选了四种绿色海藻（刚毛藻、脆松藻）的粗提物。tomentosoides、石莼和石莼（Ulva lactuca）。在体外试验中，将布氏锥虫、克氏锥虫、杜氏利什曼原虫和结核分枝杆菌用作试验微生物。还测定了提取物对哺乳动物骨骼肌成肌细胞（L 6）的选择性毒性。海藻粗提物对M.结核病，但对至少两种原生动物物种显示出抗原生动物活性。所有藻提取物对T. brucei rhodesiense和C.其中以岩藻的IC_（50）值最高（3.7 μ g/ml），而只有C. rupestris和U.莴苣对克氏锥虫具有中等的杀锥虫活性（IC 50值为80.8和34.9 μ g/ml）。同样，所有四种提取物均显示出杀利什曼原虫活性，IC 50值在12.0和20.2 μ g/ml之间。所有提取物均未表现出对L 6细胞的细胞毒性，表明其抗原虫活性具有特异性。这是第一项研究报告英国海藻的抗原生动物和抗分枝杆菌活性。版权所有（C）2009约翰威利父子有限公司
In the continuation of our search for natural sources for antiprotozoal and antitubercular molecules, we have screened the crude extracts of four green marine algae (Cladophora rupestris, Codium fragile ssp. tomentosoides, Ulva intestinalis and Ulva lactuca) collected from the Dorset area of England. Trypanosoma brucei rhodesiense, Trypanosoma cruzi, Leishmania donovani and Mycobacterium tuberculosis were used as test organisms in the in vitro assays. The selective toxicity of the extracts was also determined toward mammalian skeletal myoblast (L6) cells. The crude seaweed extracts had no activity against M. tuberculosis, but showed antiprotozoal activity against at least two protozoan species. All algal extracts were active against T. brucei rhodesiense, with C. rupestris being the most potent one (IC50 value 3.7 mu g/ml), whilst only C. rupestris and U. lactuca had moderate trypanocidal activity against T cruzi (IC50 values 80.8 and 34.9 mu g/ml). Again, all four extracts showed leishmanicidal activity with IC50 values ranging between 12.0 and 20.2 mu g/ml. None of the extracts showed cytotoxicity toward L6 cells, indicating that their antiprotozoal activity is specific. This is the first study reporting antiprotozoal and antimycobacterial activity of British marine algae. Copyright (C) 2009 John Wiley & Sons, Ltd.