Effect of Piezo1 Overexpression on Peritumoral Brain Edema in Glioblastomas
Effect of Piezo1 Overexpression on Peritumoral Brain Edema in Glioblastomas
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DOI：
10.3174/ajnr.a6638
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发表时间：
2020-07
影响因子：
3.5
通讯作者：
Shanqiang Qu;Tianyu Hu;Ouwen Qiu;Yuejiao Su;Jiayu Gu;Zhibo Xia
中科院分区：
文献类型：
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作者：
Shanqiang Qu;Tianyu Hu;Ouwen Qiu;Yuejiao Su;Jiayu Gu;Zhibo Xia
背景和目的：先前的研究表明，脑肿瘤患者死亡率和残疾率的增加与瘤周脑水肿有关。然而，脑肿瘤瘤周脑水肿的机制尚不清楚。本研究旨在探讨Piezo 1过表达对胶质母细胞瘤瘤周脑水肿的影响。材料和方法：采用定量逆转录聚合酶链反应、Western blot和免疫组化方法检测Piezo 1在细胞系和配对标本中的表达。对64例胶质母细胞瘤患者进行回顾性分析。免疫组化法检测肿瘤组织中Piezo 1的表达。术前MRI测量肿瘤直径和水肿直径，计算水肿指数值。研究结果：Western blot和定量逆转录聚合酶链反应显示Piezo 1在6种胶质瘤细胞系中的表达均高于正常星形胶质细胞系。与癌旁组织相比，Piezo 1在肿瘤组织中表达上调。64例胶质母细胞瘤患者入组进一步研究。Piezo 1在中度水肿组中高于轻度水肿组（P < .001），在重度水肿组中高于中度水肿组（P < .001），并与水肿指数相关（r = 0.73; P < .001）。受试者工作特征曲线分析显示，水肿指数的曲线下面积为0.867（95%CI，0.76-0.97; P <0.001），敏感性为100%，特异性为70%。结论：Piezo 1过表达与胶质母细胞瘤瘤周脑水肿程度呈正相关。根据水肿程度预测肿瘤组织中Piezo 1的高表达具有良好的敏感性和特异性。
BACKGROUND AND PURPOSE: Previous studies have suggested that increased mortality and disability in patients with brain tumor are associated with peritumoral brain edema. However, the mechanism of peritumoral brain edema in brain tumors is unknown. This study aimed to investigate the effect of Piezo1 overexpression on peritumoral brain edema in glioblastomas. MATERIALS AND METHODS: The Piezo1 expression in cell lines and paired samples was detected by quantitative reverse transcription polymerase chain reaction, Western blot, and immunohistochemistry. Sixty-four patients with glioblastomas were analyzed retrospectively. The Piezo1 expression of tumor tissue was detected by immunohistochemistry. The diameters of tumor and edema were measured by preoperative MR imaging, and the edema index value was calculated. RESULTS: Western blot and quantitative reverse transcription polymerase chain reaction showed that Piezo1 expression was higher in 6 glioma cell lines than in the normal astrocyte cell line. Compared with peritumoral tissues, Piezo1 was up-regulated in tumor tissues. Sixty-four patients with glioblastomas were enrolled in further study. Piezo1 was higher in the moderate edema group than in the mild edema group (P < .001), higher in the severe edema group than in the moderate edema group (P < .001), and correlated with the edema index (r = 0.73; P < .001). Receiver operating characteristic curve analysis showed that the edema index yielded an area under the curve of 0.867 (95% CI, 0.76–0.97; P < .001), with a sensitivity of 100% and a specificity of 70%. CONCLUSIONS: Piezo1 overexpression is positively correlated with the degree of peritumoral brain edema in glioblastomas. Predicting high Piezo1 expression in tumor tissues based on the edema extent shows good sensitivity and specificity.