A role for an endosperm-localized subtilase in the control of seed size in legumes
A role for an endosperm-localized subtilase in the control of seed size in legumes
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DOI：
10.1111/j.1469-8137.2012.04296.x
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发表时间：
2012-11-01
期刊：
影响因子：
9.4
通讯作者：
Gallardo, K.
中科院分区：
文献类型：
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作者：
D'Erfurth, I.;Le Signor, C.;Gallardo, K.
在这里，我们报告了一个枯草杆菌酶基因（SBT1.1）特异性表达的胚乳中的蒺藜苜蓿和豌豆种子在发展过程中，这是位于染色体上的位置相一致的种子重量的数量性状位点（QTL）。SBT1.1多态性和种子重量的生态型收集之间的关联研究提供了进一步的证据SBT1.1位点和种子重量位点之间的连锁不平衡。为了研究SBT1.1对控制种子重量的可能贡献，进行了TILLING（靶向诱导的基因组局部损伤）突变体的搜索。种子表型的检查显示，SBT1.1突变体种子的重量和面积减少，从而推断SBT1.1在控制牧草和谷类豆科植物种子大小中的作用。对代表种子主要部分的胚进行显微镜分析，发现MtP330S突变体中细胞数量减少，但细胞大小无显著变化。因此，SBT1.1最有可能参与种子发育过程中子叶细胞数量的控制，而不是细胞扩增。这提出了SBT1.1在调节种子大小中的作用的假设，通过提供可以作为控制胚胎内细胞分裂的信号的分子。
Here, we report a subtilase gene (SBT1.1) specifically expressed in the endosperm of Medicago truncatula and Pisum sativum seeds during development, which is located at a chromosomal position coinciding with a seed weight quantitative trait locus (QTL). Association studies between SBT1.1 polymorphisms and seed weights in ecotype collections provided further evidence for linkage disequilibrium between the SBT1.1 locus and a seed weight locus. To investigate the possible contribution of SBT1.1 to the control of seed weight, a search for TILLING (Targeting Induced Local Lesions in Genomes) mutants was performed. An inspection of seed phenotype revealed a decreased weight and area of the sbt1.1 mutant seeds, thus inferring a role of SBT1.1 in the control of seed size in the forage and grain legume species. Microscopic analyses of the embryo, representing the major part of the seed, revealed a reduced number of cells in the MtP330S mutant, but no significant variation in cell size. SBT1.1 is therefore most likely to be involved in the control of cotyledon cell number, rather than cell expansion, during seed development. This raises the hypothesis of a role of SBT1.1 in the regulation of seed size by providing molecules that can act as signals to control cell division within the embryo.