Response of ammonia oxidizing archaea and bacteria to decabromodiphenyl ether and copper contamination in river sediments.
Response of ammonia oxidizing archaea and bacteria to decabromodiphenyl ether and copper contamination in river sediments.
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DOI：
10.1016/j.chemosphere.2017.10.067
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发表时间：
2018
期刊：
影响因子：
8.8
通讯作者：
Linqiong Wang;Yi Li;Lihua Niu;Wenlong Zhang;Huanjun Zhang;Longfei Wang;Peifang Wang
中科院分区：
文献类型：
--
作者：
Linqiong Wang;Yi Li;Lihua Niu;Wenlong Zhang;Huanjun Zhang;Longfei Wang;Peifang Wang
氨氧化在河流氮素循环生态系统中起着基础性的作用，通常由氨氧化古生菌(AOA)和氨氧化细菌(AOB)共同控制。典型新兴污染物多溴联苯醚(PBDEs)和重金属对河流沉积物中AOA和AOB群落的复合污染尚不清楚。本研究利用高通量焦磷酸测序和实时定量聚合酶链式反应(QPCR)等多种分析手段，研究了河流沉积物中AOA和AOB的氨单加氧酶(AMO)活性、AmoA基因丰度和群落结构。结果表明，随着十溴二苯醚(BDE209，1~100m g/kg−1)和铜(Cu50~500m g/kg−1)浓度的增加，对AMO活性的抑制作用增强。此外，BDE209和铜的协同作用导致AMO活性降低的程度高于单独污染BDE209。在20g/kg−1BDE 209和100g/kg−1Cu混合污染下，AOAamoA拷贝数分别下降了75.9%和83.2%，AOBamoA基因丰度分别下降了82.8%和90.0%。焦磷酸测序结果表明，AOB和AOA群落结构都发生了变化，其中AOB的变化幅度大于AOA。结果表明，AOB微生物群落可能对BDE209和铜污染有较好的适应能力，而AOA微生物群落可能具有更强的抗逆能力。我们的研究为更好地了解重金属和微污染物联合暴露对河流沉积物中AOA和AOB的生态毒理效应提供了更好的依据。
Ammonia oxidation plays a fundamental role in river nitrogen cycling ecosystems, which is normally governed by both ammonia oxidizing archaea (AOA) and ammonia oxidizing bacteria (AOB). Co-contamination of typical emerging pollutant Polybrominated diphenyl ethers (PBDEs) and heavy metal on AOA and AOB communities in river sediments remains unknown. In this study, multiple analytical tools, including high-throughput pyrosequencing and real-time quantitative PCR (qPCR), were used to reveal the ammonia monooxygenase (AMO) activity, subunit alpha (amoA) gene abundance, and community structures of AOA and AOB in river sediments. It was found that the inhibition of AMO activities was increased with the increase of decabromodiphenyl ether (BDE 209, 1–100 mg kg−1) and copper (Cu, 50–500 mg kg−1) concentrations. Moreover, the synergic effects of BDE 209 and Cu resulted in a higher AMO activity reduction than the individual pollutant BDE 209. The AOAamoA copy number declined by 75.9% and 83.2% and AOBamoA gene abundance declined 82.8% and 90.0% at 20 and 100 mg kg−1BDE 209 with a 100 mg kg−1Cu co-contamination, respectively. The pyrosequencing results showed that both AOB and AOA community structures were altered, with a higher change of AOB than that of AOA. The results demonstrated that the AOB microbial community may be better adapted to BDE 209 and Cu pollution, while AOA might possess a greater capacity for stress resistance. Our study provides a better understanding of the ecotoxicological effects of heavy metal and micropollutant combined exposure on AOA and AOB in river sediments.