Laboratory Maintenance and Growth of Talaromyces marneffei.
Laboratory Maintenance and Growth of Talaromyces marneffei.
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DOI：
10.1002/cpmc.97
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发表时间：
2020-01-01
影响因子：
--
通讯作者：
Andrianopoulos, Alex
中科院分区：
文献类型：
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作者：
Andrianopoulos, Alex
马尔尼菲篮状菌是东南亚地区特有的一种重要的人类机会致病菌。它是一种具有热控制二型性的病原真菌。在25°C时，T.马尔尼菲菌以多细胞丝状菌丝形式生长，其可以分化产生称为分生孢子的休眠孢子。这些分生孢子很可能是传染源。在37°C时，T. marneffei生长为通过裂变分裂的单核酵母。酵母细胞是这种真菌的致病形式。这里描述的协议解释了如何培养T。marneffei在体外以两种营养生长形式生长，在哺乳动物巨噬细胞内生长酵母细胞，产生分生孢子原种，并短期和长期储存菌株。© 2020 by John Wiley & Sons，Inc.基本方案1：营养菌丝形式在固体培养基上的生长替代方案1：营养菌丝形式在液体悬浮液中的生长基本方案2：营养酵母形式在固体培养基上的生长替代方案2：营养酵母形式在液体悬浮液中的生长基本方案3：用于生产休眠分生孢子的生长支持方案：制备Miracloth过滤管基本方案4：基本方案5：马尔尼菲篮状菌菌株的储存替代方案3：马尔尼菲篮状菌菌株的冻干。
Talaromyces marneffei is an important opportunistic human pathogen endemic to Southeast Asia. It is one of a number of pathogenic fungi that exhibits thermally controlled dimorphism. At 25°C, T. marneffei grows in a multicellular, filamentous hyphal form that can differentiate to produce dormant spores called conidia. These conidia are the likely infectious agent. At 37°C, T. marneffei grows as a uninucleate yeast that divides by fission. The yeast cells are the pathogenic form of this fungus. The protocols described here explain how to grow T. marneffei in the two vegetative growth forms in vitro, grow yeast cells inside mammalian macrophages, produce conidial stocks, and store strains both short and long term. © 2020 by John Wiley & Sons, Inc. Basic Protocol 1: Growth of the vegetative hyphal form on solid medium Alternate Protocol 1: Growth of the vegetative hyphal form in liquid suspension Basic Protocol 2: Growth of the vegetative yeast form on solid medium Alternate Protocol 2: Growth of the vegetative yeast form in liquid suspension Basic Protocol 3: Growth for production of dormant conidia Support Protocol: Preparation of Miracloth filter tubes Basic Protocol 4: Growth of Talaromyces marneffei in mammalian macrophages Basic Protocol 5: Storage of Talaromyces marneffei strains Alternate Protocol 3: Lyophilization of Talaromyces marneffei strains.