Osteoclasts secrete the chemotactic cytokine mim-1
Osteoclasts secrete the chemotactic cytokine mim-1
复制标题
DOI：
10.1006/bbrc.2001.4307
复制
发表时间：
2001-02-16
影响因子：
3.1
通讯作者：
Williams, JP
中科院分区：
文献类型：
--
作者：
Falany, ML;Thames, AM;Williams, JP
破骨细胞是起源于单核细胞的终末分化的多核细胞。在这项研究中，我们报告破骨细胞分泌一种35kD的蛋白质，佛波醇肉豆蔻酸醋酸酯处理显著刺激分泌。用质谱仪分析了蛋白质的多肽酶切图谱。该蛋白被鉴定为MYB诱导的髓样蛋白-1前体(mim-1蛋白)。MIM-1由造血细胞特异性表达，功能未知。它与中性粒细胞趋化因子软骨调制素II同源，后者刺激成骨细胞和软骨细胞的增殖。Western分析显示，破骨细胞分泌mim-1进入培养液。免疫荧光研究表明，mim-1在禽类破骨细胞和人破骨细胞样细胞中均有胞浆和核周分布。破骨细胞表达和分泌趋化素样蛋白提示了骨微环境中一种新的信号通路，可能参与协调骨重建。(C)2001年学术出版社。
Osteoclasts are terminally differentiated, multinucleated cells of monocytic origin. In this study, we report that osteoclasts secrete a 35 kD protein and that phorbol myristate acetate treatment stimulates secretion dramatically. Peptide digests of the protein were analyzed by mass spectroscopy. The protein was identified as myb induced myeloid protein-1 precursor (mim-1 protein). Mim-1 is expressed specifically by hematopoietic cells and has no known function. It is homologous with the neutrophil chemokine, chondromodulin II, which stimulates proliferation of osteoblasts and chondrocytes. Western analysis showed that osteoclasts secrete mim-1 into culture media. Immunofluorescence studies demonstrated an cytoplasmic and perinuclear distribution of mim-1 in both avian osteoclasts and human osteoclast-like cells. Expression and secretion of a chemokine-like protein by osteoclasts suggests a novel signaling pathway in the bone microenvironment that may be involved in coordinating bone remodeling. (C) 2001 Academic Press.