Assessment of using Laponite® cross-linked poly(ethylene oxide) for controlled cell adhesion and mineralization
Assessment of using Laponite® cross-linked poly(ethylene oxide) for controlled cell adhesion and mineralization
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DOI：
10.1016/j.actbio.2010.09.015
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发表时间：
2011-02-01
影响因子：
9.7
通讯作者：
Schmidt, Gudrun
中科院分区：
文献类型：
--
作者：
Gaharwar, Akhilesh K.;Schexnailder, Patrick J.;Schmidt, Gudrun
以MC3T3-E1小鼠成骨前细胞为材料，研究了硅酸盐（拉脱石(R)粘土）交联聚环氧乙烷（PEO）纳米复合膜的体外细胞相容性，并评价了膜成分对细胞粘附、扩散、增殖和矿化的影响。通过将PEO聚合物的优势特性（亲水性，防止蛋白质和细胞粘附）与合成层状硅酸盐的优势特性（带电，可降解和潜在的生物活性）相结合，可以控制所得到的聚合物纳米复合材料的一些物理和化学特性。考察了水化、溶解和力学性能及其与细胞粘附的关系。总的来说，这项可行性研究证明了使用拉脱石交联PEO纳米复合材料模型来制造生物活性支架的能力。(C) 2010材料学报Elsevier Ltd.出版。版权所有。
The in vitro cytocompatibility of silicate (Laponite (R) clay) cross-linked poly(ethylene oxide) (PEO) nanocomposite films using MC3T3-E1 mouse preosteoblast cells was investigated while cell adhesion, spreading, proliferation and mineralization were assessed as a function of film composition. By combining the advantageous characteristics of PEO polymer (hydrophilic, prevents protein and cell adhesion) with those of a synthetic and layered silicate (charged, degradable and potentially bioactive) some of the physical and chemical properties of the resulting polymer nanocomposites could be controlled. Hydration, dissolution and mechanical properties were examined and related to cell adhesion. Overall, this feasibility study demonstrates the ability of using model Laponite cross-linked PEO nanocomposites to create bioactive scaffolds. (C) 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.