Degradation of pyrene by Mycobacterium flavescens
Degradation of pyrene by Mycobacterium flavescens
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DOI：
10.1007/s002530050822
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发表时间：
1996-10-01
影响因子：
5
通讯作者：
Cerniglia, CE
中科院分区：
文献类型：
--
作者：
DeanRoss, D;Cerniglia, CE
从受污染的底泥中分离到一株黄色分枝杆菌。它能够利用芘作为碳和能量的唯一来源。当芘以悬浮液的形式以50 μ g/ml的浓度提供时，世代时间为9.6小时，芘的利用率为0.56 μ g/ml/天。除了芘，菌株可以矿化菲（17.7%）和荧蒽（17.9%），但不能矿化萘，蒽，蒽，芴，苊和苯并[a]芘，如通过在生长条件下进行的2周孵育中的放射性标记的CO2的回收测定。通过HPLC和GC-MS对芘生长过程中产生的代谢产物进行了检测和表征，确定了初始环氧化产物4，5-二羟基-4，5-二氢芘，以及环分裂产物4-菲咯酸、邻苯二甲酸和4，5-菲二酸。
A strain of Mycobacterium flavescens was isolated from polluted sediments. It was capable of utilizing pyrene as a sole source of carbon and energy. When pyrene was supplied as a suspension at 50 mu g/ml, the generation time was 9.6 h and the rate of pyrene utilization was 0.56 mu g ml(-1) day(-1). In addition to pyrene, the strain could mineralize phenanthrene (17.7%) and fluoranthene (17.9%), but failed to mineralize naphthalene, chrysene, anthracene, fluorene, acenaphthene and benzo[a]pyrene, as determined by recovery of radio-labeled CO2 in incubations conducted for 2 weeks under growth conditions. Metabolites produced during growth on pyrene were detected and characterized by HPLC and GC-MS. The product of initial ring oxidation, 4,5-dihydroxy-4,5-dihydropyrene was identified, as well as ring-fission products including 4-phenanthroic acid, phthalic acid, and 4,5-phenanthrenedioic acid.