Pore size distributions of ion exchangers and relation to protein binding capacity.
Pore size distributions of ion exchangers and relation to protein binding capacity.
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DOI：
10.1016/j.chroma.2006.06.057
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发表时间：
2006-09
期刊：
影响因子：
--
通讯作者：
Yan Yao;A. Lenhoff
中科院分区：
文献类型：
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作者：
Yan Yao;A. Lenhoff
色谱介质的孔结构直接影响大分子的传输和吸附，从而影响色谱分离中的分离分辨率和负载能力。因此，孔径分布（PSD）是色谱材料的核心结构特征，也是色谱行为的关键决定因素。本文采用反相体积排阻色谱法测定了一系列商品阴离子交换剂的PSD。进一步利用PSD开发的吸附剂的功能特性，如颗粒内扩散，静态和动态结合能力的关系。我们发现，详细的PSD是有用的半定量的理解色谱行为。然而，更准确地预测柱行为需要更全面地了解孔结构，特别是孔网络的连通性，以及对接枝树脂功能的更好理解。
The pore structure of chromatographic media directly influences macromolecular transport and adsorption, and consequently separation resolution and loading capacity in chromatographic separations. The pore size distribution (PSD) is therefore a central structural characteristic of chromatographic materials and a critical determinant of chromatographic behavior. In this work the PSDs of a set of commercial anion exchangers were determined by inverse size-exclusion chromatography (ISEC). The PSDs were further utilized to develop relations to functional properties of adsorbents, such as intraparticle diffusivity, and static and dynamic binding capacities. We find that the detailed PSD is useful in semi-quantitative understanding of chromatographic behavior. However, more accurate prediction of column behavior requires more thorough knowledge of the pore structure, specifically the connectivity of the pore network, as well as improved understanding of the function of grafted resins.