Stimulation of the hexose monophosphate shunt in bovine ciliary body under oxidative stress.
Stimulation of the hexose monophosphate shunt in bovine ciliary body under oxidative stress.
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氧化应激下牛睫状体己糖单磷酸分流的刺激。
DOI：
10.1089/jop.1986.2.59
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Giblin,FJ
中科院分区：
文献类型：
--
作者：
Shichi,H;Hodder,WA;Giblin,FJ
在谷胱甘肽还原酶抑制剂呋喃妥因(NF)和1，3-二(2-氯乙基)-1-亚硝脲[BCNU]存在和不存在的情况下，研究了叔丁基氢过氧化氢(TBHP)对牛睫状体氧化标记的C_1和C_6标记葡萄糖的影响。使用组织提取物，BCNU被证明同时抑制谷胱甘肽还原酶和葡萄糖-6-磷酸脱氢酶。这些结果支持牛睫状体通过己糖一磷酸分流具有较高的NADPH再生能力的假设。
The effect of t-butyl hydroperoxide (TBHP) on the oxidation of C1-radiolabeled glucose and C6-radiolabeled glucose by bovine ciliary body was investigated in the presence and absence of the glutathione reductase inhibitors nitrofurantoin (NF) and 1,3-bis(2-chloroethyl)-1-nitrosourea [BCNU].14CO2production from 1-14C-glucose was markedly stimulated by TBHP and inhibited by BCNU. Using tissue extracts, BCNU was shown to inhibit both glutathione reductase and glucose-6-phosphate dehydrogenase. These results support the hypothesis that bovine ciliary body has high NADPH regenerating capacity by the hexose monophosphate shunt.