Solution Structure of an Arabidopsis WRKY DNA Binding Domainw⃞
Solution Structure of an Arabidopsis WRKY DNA Binding Domainw⃞
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DOI：
10.1105/tpc.104.026435
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发表时间：
2005-03
期刊：
影响因子：
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通讯作者：
K. Yamasaki;T. Kigawa;M. Inoue;M. Tateno;T. Yamasaki;T. Yabuki;M. Aoki;Eiko Seki;T. Matsuda
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文献类型：
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作者：
K. Yamasaki;T. Kigawa;M. Inoue;M. Tateno;T. Yamasaki;T. Yabuki;M. Aoki;Eiko Seki;T. Matsuda
WRKY蛋白是一类重要的转录因子家族，在高等植物对病原菌和水杨酸的反应以及多种植物特异性反应中发挥重要作用。它们共有一个DNA结合结构域，称为WRKY结构域，其中包含一个不变的WRKYGQK序列和一个CX 4 - 5CX 22 - 23 HXH锌结合基序。在这里，我们报告的C-末端WRKY结构域的拟南芥WRKY 4蛋白的NMR溶液结构。该结构由一个四链β-折叠组成，其中一个锌结合口袋由位于β-折叠一端的保守Cys/His残基形成，揭示了一种新的锌与DNA结合结构。WRKYGQK残基对应于最N端的β链，在序列中间通过Gly残基扭结，这使得能够实现涉及Trp残基的广泛疏水相互作用，并有助于β折叠的结构稳定性。基于核磁共振化学位移扰动的配置文件，我们建议，同一条链进入DNA凹槽，并与DNA碱基形成接触。
The WRKY proteins comprise a major family of transcription factors that are essential in pathogen and salicylic acid responses of higher plants as well as a variety of plant-specific reactions. They share a DNA binding domain, designated as the WRKY domain, which contains an invariant WRKYGQK sequence and a CX4–5CX22–23HXH zinc binding motif. Herein, we report the NMR solution structure of the C-terminal WRKY domain of the Arabidopsis thaliana WRKY4 protein. The structure consists of a four-stranded β-sheet, with a zinc binding pocket formed by the conserved Cys/His residues located at one end of the β-sheet, revealing a novel zinc and DNA binding structure. The WRKYGQK residues correspond to the most N-terminal β-strand, kinked in the middle of the sequence by the Gly residue, which enables extensive hydrophobic interactions involving the Trp residue and contributes to the structural stability of the β-sheet. Based on a profile of NMR chemical shift perturbations, we propose that the same strand enters the DNA groove and forms contacts with the DNA bases.