Expression of PDZ-binding kinase/T-LAK cell-originated protein kinase (PBK/TOPK) in human urinary bladder transitional cell carcinoma
Expression of PDZ-binding kinase/T-LAK cell-originated protein kinase (PBK/TOPK) in human urinary bladder transitional cell carcinoma
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DOI：
10.1016/j.imbio.2014.02.003
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发表时间：
2014-06-01
期刊：
影响因子：
2.8
通讯作者：
Bhatt, M. L. B.
中科院分区：
文献类型：
--
作者：
Singh, P. K.;Srivastava, Anupam K.;Bhatt, M. L. B.
为探讨PDZ结合蛋白/T-LAK细胞源性蛋白激酶(PBK/TOPK)在膀胱癌组织中的表达及其临床意义，采用RT-PCR方法检测PBK/TOPK在膀胱癌及正常睾丸组织中的表达。定量逆转录聚合酶链式反应显示PBK/TOPK在BC组织中的表达高于癌旁组织(ANCT)(p<；0.0001)。PBK/TOPK蛋白胞浆表达阳性率为64.6%(42/65)。肌肉浸润性膀胱癌组织中pBK/TOPK蛋白的表达显著高于非肌肉浸润性膀胱癌组织(86.1%vs.37.9%，p<；0.001)。PBK/TOPK的免疫组织化学(IHC)表达与疾病的分期显著相关(p<；0.001)。研究结果表明，pBK/TOPK的表达具有人BC的特异性，可作为肿瘤免疫治疗和诊断生物标记物的新靶点。(C)2014年爱思唯尔股份有限公司。版权所有。
The objective of this study was to evaluate the expression pattern of PDZ-binding kinase/T-LAK cell-originated protein kinase (PBK/TOPK) and its clinical significance in human bladder cancer (BC).We detected PBK/TOPK mRNA overexpression in BC and human normal testis tissues using RT-PCR. Using qRT-PCR revealed a higher expression of PBK/TOPK in BC tissues than their adjacent noncancerous tissues (ANCTs) (p < 0.0001). Cytoplasmic expression of PBK/TOPK protein was found to be positive in 64.6% (42 of 65) BC patients. Expression of PBK/TOPK protein was found to be significantly higher in muscle-invasive bladder cancer (MIBC) than in non-muscle-invasive bladder cancer (NMIBC) (86.1% vs. 37.9%, p < 0.001). The immunohistochemical (IHC) expression of PBK/TOPK was found to be significantly (p < 0.001) associated with the stage of disease.Study findings suggest that the PBK/TOPK mRNA/protein expression is specific to human BC and might be used as a novel target for development of cancer immunotherapy and diagnostic biomarker. (C) 2014 Elsevier GmbH. All rights reserved.