Can HPV-16 genotyping provide a benchmark for cervical biopsy specimen interpretation?
Can HPV-16 genotyping provide a benchmark for cervical biopsy specimen interpretation?
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DOI：
10.1309/a8mfc18twanc8qfh
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发表时间：
2008-07-01
影响因子：
3.5
通讯作者：
Schiffman, Mark
中科院分区：
文献类型：
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作者：
Galgano, Mary T.;Castle, Philip E.;Schiffman, Mark
人乳头瘤病毒(HPV)检测是宫颈细胞学检查的客观质量保证基准。尽管宫颈活检具有相似的观察者间可变性，但没有可比较的指标。由于HPV-16阳性率随宫颈病变的严重程度增加而增加，因此我们探讨了HPV-16检测是否可以作为区分宫颈上皮内瘤变(CIN)2级和CIN 2级或更差(代表治疗的临床阈值)的有用指标。通过使用意义不确定的非典型鳞状细胞-低度鳞状上皮内病变分流研究数据，我们比较了10名临床中心(CC)病理学家和质量控制(QC)组的活检诊断。尽管HPV-16的百分比与。诊断的严重性，每个CC病理学家之间的比率有很大的差异。个别CC病理学家和QC组对含有HPV-16的CIN 2或更差的百分比的一致性与诊断是否低于CIN 2或CIN 2或更差的一致性弱相关。因此，在这项分析中，没有发现HPV-16组分作为活检诊断的质量保证指标广泛有用。
Human papillomavirus (HPV) detection is an objective quality assurance benchmark for cervical cytology. There is no comparable metric for cervical biopsies despite biopsies having similar interobserver variability. Because HPV-16 positivity increases with the severity of cervical abnormality, we explored whether HPV-16 detection might be a useful metric for distinguishing a diagnosis of less than cervical intraepithelial neoplasia (CIN) 2 from CIN 2 or worse (representing the clinical threshold for treatment). By using Atypical Squamous Cells of Undetermined Significance-Low-Grade Squamous Intraepithelial Lesion Triage Study data, we compared biopsy diagnoses of the 10 clinical center (CC) pathologists with the quality control (QC) group. Although the percentage of HPV-16 correlated with. severity of diagnoses, there was great variability between the rates for each individual CC pathologist. Agreement between individual CC pathologists and the QC group on the percentage of CIN 2 or worse containing HPV-16 correlated weakly with agreement on whether the diagnosis was less than CIN 2 or CIN 2 or worse. Thus the HPV-16 fraction was not found to be broadly useful as a quality assurance metric for biopsy diagnosis in this analysis.