Distribution and quantitative analysis of CIDEa and CIDEc in broiler chickens: accounting for differential fat deposition between strains
Distribution and quantitative analysis of CIDEa and CIDEc in broiler chickens: accounting for differential fat deposition between strains
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肉鸡中 CIDEa 和 CIDEc 的分布和定量分析：解释品系之间脂肪沉积的差异
DOI：
10.1080/00071668.2017.1415426
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发表时间：
2018-01-01
影响因子：
2
通讯作者：
Ruan, J. M.
中科院分区：
文献类型：
--
作者：
Huang, J. Z.;Huang, L. M.;Ruan, J. M.
摘要1.研究了CIDEa和CIDEc在20种不同组织中的表达差异。CIDEa和CIDEc基因转录本主要在白色脂肪组织(WAT)中表达，但在肾脏和肝脏中也有中等水平的表达，在卵巢中也有较低水平的表达。有趣的是，在WAT类型中，CIDEa和CIDEc在心脏冠状Wat中的表达水平最低。2.为了更好地了解CIDEa和CIDEc在肉鸡脂肪沉积中的作用，研究了瘦肉型和肥胖型肉鸡的脂滴大小和CIDEa和CIDEc基因表达水平的差异。肥胖型肉鸡的脂肪体积较大，白色脂肪、肾脏和肝脏组织中CIDEa和CIDEc的mRNA水平显著高于瘦肉型肉鸡。3.分析了Arbor Acres肉鸡不同组织类型(Wat、肝脏和肾脏)中CIDEa和CIDEc基因的表达模式，发现CIDEa和CIDEc基因的表达水平在不同发育阶段逐渐升高，在6.4周龄达到最高水平。这些观察结果表明，CIDEa和CIDEc的功能反映了脂肪代谢的内在特征，导致了品系间脂肪沉积的差异。本研究的结果有助于更好地了解CIDEa和CIDEc基因的组织分布和表达模式，并有助于进一步研究肉鸡脂肪沉积的分子机制。
ABSTRACT 1. Differences in the expression of CIDEa and CIDEc in 20 different tissues were examined. Both CIDEa and CIDEc mRNA transcripts were predominantly but variably expressed in white adipose tissue (WAT) but were also expressed at moderate levels in the kidney and liver and at lower levels in the ovary. Interestingly, among WAT types, both CIDEa and CIDEc were expressed at the lowest levels in heart coronary WAT. 2. To better understand the roles of CIDEa and CIDEc in the fat deposition of broiler chickens, the differences in lipid droplet (LD) size and mRNA levels of CIDEa and CIDEc between lean-type and fat-type broiler chicken lines were studied. LD sizes were larger in fat-type broiler lines, and CIDEa and CIDEc mRNA levels in white adipose, kidney and liver tissues were significantly higher in fat-type broiler lines than in their lean counterparts. 3. Developmental expression patterns of CIDEa and CIDEc mRNA were analysed in different tissue types (WAT, liver and kidney) in Arbor Acres broiler chickens, and CIDEa and CIDEc mRNA expression levels increased during sequential developmental stages, achieving peak expression levels at week 6. 4. These observations suggest that the functions of CIDEa and CIDEc reflect inherent characteristics of lipid metabolism that contribute to the differences in fat deposition between strains. The results in this study contribute to a more robust understanding of the tissue distribution and expression patterns of CIDEa and CIDEc mRNA and facilitate further research concerning the molecular mechanism underlying fat deposition in broiler chickens.