Sensitive dual-labeled electrochemical aptasensor for simultaneous detection of multi-antibiotics in milk
Sensitive dual-labeled electrochemical aptasensor for simultaneous detection of multi-antibiotics in milk
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DOI：
10.1016/j.ijhydene.2021.04.007
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发表时间：
2021-06
影响因子：
7.2
通讯作者：
Falan Li;Yanfang Wu;Dongfei Chen;Yemin Guo;Xiangyou Wang;Xia Sun
中科院分区：
文献类型：
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作者：
Falan Li;Yanfang Wu;Dongfei Chen;Yemin Guo;Xiangyou Wang;Xia Sun
本研究以卡那霉素（KAN）和妥布霉素（tobramycin）为模型分析物，构建了可同时检测多种抗生素的双标记多重适体传感器。本文报道的适体传感器具有三个关键要素，即基于rna的适体链、半导体量子点（QDs）和金纳米壳（auns）。由于链亲和素（SA）和生物素（Bio）之间的高亲和力配对，将两个生物素化的适配体（卡那霉素适配体KAP和妥布霉素适配体TAP）分别偶联到SA包覆的硫化镉（CdS）和硫化铅（PbS）量子点上，合成了KAP-Bio-SA-CdS和TAP-Bio-SA- pds复合物。此外，由于其优异的表面积体积比，aass在金电极表面对制备的两种复合材料具有非常高的负载效率。在目标抗生素存在的情况下，CdS和PbS量子点从aass中释放出来，然后溶解到各自的金属离子中，用差分脉冲伏安法（DPV）进行溶出分析。结果，由于大量的金属离子标签溶解在溶液中，从DPV峰获得了增强的信号。与已有的传感器相比，所设计的传感器具有更宽的检测线性范围（KAN, 1-4 × 102nM； TOB, 1-1 × 104nM）和更低的检出限（KAN, 0.12 nM； TOB, 0.49 nM）。此外，还成功地验证了在牛奶样品中添加KAN和TOB的方法。因此，该传感器为多种抗生素的检测提供了一个新的灵敏平台。
In this work, a dual-labeled multiple aptasensor that can simultaneously detect multiple antibiotics was constructed, with kanamycin (KAN) and tobramycin (TOB) used as the model analytes. The aptasensor reported here featured three key elements, namely the RNA-based aptamer strands, semiconductor quantum dots (QDs), and gold nanoshells (AuNSs). Due to the high-affinity pairing between streptavidin (SA) and biotin (Bio), the two biotinylated aptamers (kanamycin aptamer, KAP; tobramycin aptamer, TAP) responsible for the specific recognition of KAN and TOB were conjugated to SA-coated cadmium sulfide (CdS) and lead sulfide (PbS) QDs, respectively for the syntheses of KAP-Bio-SA-CdS and TAP-Bio-SA-PdS composites. Moreover, the AuNSs had a very high loading efficiency for the as-prepared two composites on the gold electrode surface due to their outstanding surface-area-to-volume ratio. In the presence of target antibiotics, CdS and PbS QDs were released from AuNSs followed by dissolution into their respective metal ions for stripping analysis by differential pulse voltammetry (DPV). As a result, intensified signals from DPV peaks were obtained due to the large amount of the metal ion labels dissolved in the solution. The devised aptasensor exhibited wider detection linear range (KAN, 1–4 × 102nM; TOB, 1–1 × 104nM) and lower detection limits (KAN, 0.12 nM; TOB, 0.49 nM) compared to the previously reported sensors. Furthermore, the detection of KAN and TOB spiked in milk samples was successfully demonstrated. Therefore, the proposed aptasensor provides a novel sensitive platform for multi-antibiotics detection.