Naturally produced citral can significantly inhibit normal physiology and induce cytotoxicity on Magnaporthe grisea
Naturally produced citral can significantly inhibit normal physiology and induce cytotoxicity on Magnaporthe grisea
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天然产生的柠檬醛可显着抑制稻瘟病菌的正常生理并诱导细胞毒性
DOI：
10.1016/j.pestbp.2014.10.015
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发表时间：
2015-02-01
影响因子：
4.7
通讯作者：
Li, Ming
中科院分区：
文献类型：
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作者：
Li, Rong-Yu;Wu, Xiao-Mao;Li, Ming
鉴于寻找合成杀菌剂替代品的重要性，测定了天然产物柠檬醛对6种植物病原真菌（稻瘟病菌、玉米赤霉、尖孢镰刀菌、苹果腐烂病、灰葡萄孢和立枯丝核菌）的抗真菌作用。菌丝生长速率测定结果表明，柠檬醛对供试真菌具有较好的抑制作用，其EC 50值为39.52 ~ 193.00 μ g/mL，其中对M.稻瘟病菌柠檬醛对M.稻瘟病菌进行了研究。柠檬醛处理改变了M.稻瘟病菌通过引起细胞质的损失和菌丝体的变形而破坏菌丝。此外，柠檬醛还能诱导M.稻瘟病菌，表明细胞壁的破坏。这些结果表明，柠檬醛可能通过破坏细胞壁完整性和膜渗透性起作用，从而导致生理变化并引起细胞毒性。重要的是，柠檬醛对M.稻瘟病菌对菌丝体还原糖、可溶性蛋白、几丁质酶活性、丙酮酸含量和丙二醛含量的影响。(C)2014爱思唯尔公司All rights reserved.
Given the importance of finding alternatives to synthetic fungicides, the antifungal effects of natural product citral on six plant pathogenic fungi (Magnaporthe grisea, Gibberella zeae, Fusarium oxysporum, Valsa mali, Botrytis cinerea, and Rhizoctonia solani) were determined. Mycelial growth rate results showed that citral possessed high antifungal activities on those test fungi with EC50 values ranging from 39.52 to 193.00 mu g/mL, which had the highest inhibition rates against M. grisea. Further action mechanism of citral on M. grisea was carried out. Citral treatment was found to alter the morphology of M. grisea hyphae by causing a loss of cytoplasm and distortion of mycelia. Moreover, citral was able to induce an increase in chitinase activity in M. grisea, indicating disruption of the cell wall. These results indicate that citral may act by disrupting cell wall integrity and membrane permeability, thus resulting in physiology changes and causing cytotoxicity. Importantly, the inhibitory effect of citral on M. grisea appears to be associated with its effects on mycelia reducing sugar, soluble protein, chitinase activity, pyruvate content, and malondialdehyde content. (C) 2014 Elsevier Inc. All rights reserved.