Blood group type glycosphingolipids from the small intestine of different animals analysed by mass spectrometry and thin-layer chromatography. A note on species diversity.
Blood group type glycosphingolipids from the small intestine of different animals analysed by mass spectrometry and thin-layer chromatography. A note on species diversity.
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通过质谱和薄层色谱分析来自不同动物小肠的血型型鞘糖脂。
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
H. Leffler
中科院分区：
文献类型：
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作者：
M. Breimer;G. Hansson;K. Karlsson;H. Leffler
从猫、鳕鱼、豚鼠、母鸡、小鼠、兔和两种大鼠的小肠中分离出总非酸性鞘糖脂。通过薄层色谱法和质谱法分析样品并进行免疫活性分析。全甲基化和LiAlH 4-减少全甲基化衍生物的质谱允许的结构（碳水化合物序列和神经酰胺组合物）的解释高达9种糖脂在一个混合物。通过直接入口探针的温度编程促进了解释，导致糖脂物质主要根据糖的数量连续蒸发。在大多数情况下，所得出的结构可以归属于薄层色谱法显示的单独条带。通过免疫学分析解决了由光谱提出的抗原决定簇。因此，在猫、豚鼠、母鸡和小鼠中鉴别出Forssman糖脂，在猫、兔和大鼠中鉴别出A血型糖脂，在兔和大鼠中鉴别出B血型糖脂。没有发现刘易斯活性。某些类型的神经酰胺被证明优先存在于某些糖脂中。Globoside和Forssman糖脂（globo系列）与大多数岩藻脂和其他糖脂（两个或三个羟基）相比，羟基化神经酰胺较少（一个游离羟基）。总之，肠道糖脂模式在物种之间以及同一物种的个体之间存在差异。
The total non-acid glycosphingolipids were isolated from the small intestine of cat, cod-fish, guinea-pig, hen, mouse, rabbit, and two strains of rat. The samples were analyzed by thin-layer chromatography and mass spectrometry and for immunological activity. Mass spectrometry of permethylated and LiAlH4-reduced permethylated derivatives allowed the interpretation of the structures (carbohydrate sequence and ceramide composition) of up to 9 glycolipid species in one mixture. The interpretation was facilitated by a temperature programming of the direct inlet probe, leading to a successive evaporation of glycolipid species mainly according to the number of sugars. The structures concluded could in most cases be assigned to the separate bands revealed by thin-layer chromatography. Antigenic determinants proposed by the spectra were settled by immunological analysis. Thus, Forssman glycolipid was identified in cat, guinea-pig, hen and mouse, blood group A glycolipids in cat, rabbit, and rat and blood group B glycolipids in rabbit and rat. No Lewis activity was found. Certain ceramide types were demonstrated to exist preferentially in some glycolipids. Globoside and Forssman glycolipids (globo series) had a less hydroxylated ceramide (one free hydroxyl) compared to most fucolipids and other glycolipids (two or three hydroxyls). In conclusion, glycolipid patterns of intestine vary between species, and individuals of the same species.