Differential gene expression in the honeybee head after a bacteria challenge
Differential gene expression in the honeybee head after a bacteria challenge
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DOI：
10.1016/j.dci.2008.01.010
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发表时间：
2008-01-01
影响因子：
2.9
通讯作者：
Jacobs, Frans J.
中科院分区：
文献类型：
--
作者：
Scharlaken, Bieke;de Graaf, Dirk C.;Jacobs, Frans J.
免疫系统和神经系统之间的双向相互作用良好。建立在脊椎动物中。昆虫表现出与脊椎动物相似的神经免疫行为相互作用。使用定量实时 PCR，我们提供了证据，表明蜜蜂头部的基因表达受到大肠杆菌细菌攻击后 8 小时免疫系统激活的影响。选择七个基因进行定量分析，以涵盖头部的典型功能，如外分泌分泌（mrjp3和mrjp4）和嗅觉过程（obp17），以及更一般的过程，如结构功能（mlc2和副肌球蛋白）、应激反应（ERp60）和能量管家（烯醇化酶）。通过这种方式，我们在分子水平上证明，免疫系统在昆虫中起着感觉器官的作用，就像在脊椎动物中一样，它向头部发出信号，表明存在细菌感染，并通过一种尚未确定的机制来调节头部中几个基因的表达。 (C) 2008 Elsevier Ltd. 保留所有权利。
Bidirectional interactions between the immune and nervous systems are well. established in vertebrates. Insects show similar neuro-immune-behavioral interactions to those seen in vertebrates. Using quantitative real-time PCR, we present evidence that gene expression in the honeybee head is influenced by activation of the immune system 8 h after a bacterial challenge with Escherichia coli. Seven genes were selected for quantitative analysis in order to cover both typical functions of the head such as exocrine secretion (mrjp3 and mrjp4) and olfactory processes (obp17) as well as more general processes such as structural functions (mlc2 and paramyosin), stress response (ERp60) and energy housekeeping (enolase). In this way, we show at the molecular level that the immune system functions as a sensory organ in insects-as it does in vertebrates-which signals to the head that a bacterial infection is present, and leads to regulation of expression of several genes in the head by a yet unidentified mechanism. (C) 2008 Elsevier Ltd. All rights reserved.