Comparison of the interaction of human organic anion transporter hOAT4 with PDZ proteins between kidney cells and placental cells.
Comparison of the interaction of human organic anion transporter hOAT4 with PDZ proteins between kidney cells and placental cells.
复制标题
人有机阴离子转运蛋白 hOAT4 与肾细胞和胎盘细胞之间 PDZ 蛋白相互作用的比较。
DOI：
10.1007/s11095-007-9359-4
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发表时间：
2008
影响因子：
3.7
通讯作者：
You,Guofeng
中科院分区：
文献类型：
--
作者：
Zhou,Fanfan;Xu,Wen;Tanaka,Kunihiko;You,Guofeng
目的比较人有机阴离子转运体hOAT 4与PDZ蛋白在肾细胞和胎盘细胞中的相互作用。材料与方法将PDZ蛋白PDZK 1和NHERF 1转染表达hOAT 4或hOAT 4-Δ（缺少PDZ共有结合位点）的肾LLC-PK 1细胞和胎盘BeWo细胞。结果PDZK 1和NHERF 1通过增加hOAT 4转运蛋白的细胞表面表达，增强hOAT 4的活性。相比之下，这两个PDZ蛋白没有影响hOAT 4在BeWo cells. ConclusionPDZ蛋白与hOAT 4的相互作用可能是细胞特异性的。在胎盘中，可能需要来自PDZK 1和NHERF 1的一组不同的相互作用蛋白来调节hOAT 4活性。
PurposeTo compare the interaction of human organic anion transporter hOAT4 with PDZ proteins between kidney cells and placental cells.Materials and MethodsPDZ proteins PDZK1 and NHERF1 were transfected into kidney LLC-PK1 cells and placental BeWo cells expressing hOAT4 or hOAT4-Δ, which lacks the PDZ consensus binding site. The interaction of PDZK1 and NHERF1 with hOAT4 and hOAT4-Δ was investigated by measurement of [3H] estrone sulfate uptake, cell surface and total cell expression of hOAT4.ResultsPDZK1 and NHERF1 enhanced hOAT4 activity in LLC-PK1 cells by increasing the cell surface expression of the transporter. In contrasts, these two PDZ proteins had no effect on hOAT4 activity in BeWo cells.ConclusionThe interaction of PDZ proteins with hOAT4 may be cell-specific. In placenta, a different set of interacting proteins from PDZK1 and NHERF1 may be required to modulate hOAT4 activity.