Dynamic 3D Culture Promotes Spontaneous Embryonic Stem Cell Differentiation In Vitro
Dynamic 3D Culture Promotes Spontaneous Embryonic Stem Cell Differentiation In Vitro
复制标题
DOI：
10.1089/ten.tec.2008.0654
复制
发表时间：
2010-02-01
影响因子：
3
通讯作者：
Zeilinger, Katrin
中科院分区：
文献类型：
--
作者：
Gerlach, Joerg C.;Hout, Mariah;Zeilinger, Katrin
在一种新型的四室交织毛细管生物反应器中，通过动态三维（3D）组织密度灌注技术，连续介质灌注和交换，促进小鼠胚胎干细胞（mESC）的体外自发分化。我们比较了外胚层，内胚层和中胚层免疫反应组织结构形成的胚胎干细胞在培养第10天与小鼠胎儿组织发育在妊娠第E9.5天。结果显示，生物反应器培养物比培养在培养皿中的对照mESC更接近于妊娠E9.5天的小鼠胎儿组织发育。
Spontaneous in vitro differentiation of mouse embryonic stem cells (mESC) is promoted by a dynamic, three-dimensional (3D), tissue-density perfusion technique with continuous medium perfusion and exchange in a novel four-compartment, interwoven capillary bioreactor. We compared ectodermal, endodermal, and mesodermal immunoreactive tissue structures formed by mESC at culture day 10 with mouse fetal tissue development at gestational day E9.5. The results show that the bioreactor cultures more closely resemble mouse fetal tissue development at gestational day E9.5 than control mESC cultured in Petri dishes.