A mouse model of graded contusive spinal cord injury
A mouse model of graded contusive spinal cord injury
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DOI：
10.1089/neu.1998.15.125
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发表时间：
1998-02-01
影响因子：
4.2
通讯作者：
Wrathall, JR
中科院分区：
文献类型：
--
作者：
Kuhn, PL;Wrathall, JR
脊髓损伤（SCI）的小鼠模型可以进一步增加我们对损伤和恢复机制的基本理解，这是通过利用小鼠中天然存在的和基因工程突变来实现的。因此，我们研究了用于在大鼠中产生和评估分级实验性挫伤性SCI的方法是否可以被修改以产生创伤性SCI的小鼠模型。用2，2，2-三溴乙醇麻醉C57 BL 6小鼠，并在T8椎骨水平进行限制性椎板切除术。稳定脊柱，并使用重物下落技术产生挫伤。实验组通过重量的量或重量从其下落到位于硬脑膜上的蓄积器上的高度来区分（IG x 2.5 cm、2 g x 2.5 cm、3 g x 2.5 cm和3 g x 5.0 cm）。通过测试后肢反射反应和协调运动功能，检查SCI后28天内随时间推移的功能缺陷。慢性病变的组织病理学进行了评价，光镜和形态计量学技术进行分析。所有组在损伤后均表现出严重的功能缺陷，随后逐渐恢复。2 g x 2.5 cm组和3 g x 2.5 cm组的恢复率与重量下降和保留的白色物质百分比相关，分别为41.3 +/- 6.0%（平均值+/- SEM）和24.3 +/- 5.0%。重复实验证实了损伤的可重复性，这种新的挫伤性SCI小鼠模型可以为体内研究特定突变产生的遗传修饰对脊髓创伤后损伤和恢复过程的影响铺平道路。
A mouse model of spinal cord injury (SCI) could further increase our basic understanding of the mechanisms involved in injury and recovery by taking advantage of naturally-occurring and genetically engineered mutations available in mice. We have, therefore, investigated whether methods used to produce and evaluate graded experimental contusive SCI in the rat could be modified to produce a mouse model of traumatic SCI. C57BL6 mice were anesthetized with 2,2,2-tribromoethanol and a restricted laminectomy performed at the T8 vertebral level, The spinal column was stabilized and a weight drop technique used to produce contusive injury. Experimental groups were distinguished by the amount of weight or the height from which the weight was dropped onto an impounder resting on the dura (Ig x 2.5 cm, 2 g x 2.5 cm, 3 g x 2.5 cm, and 3 g x 5.0 cm). Functional deficits over time were examined up to 28 days after SCI by testing hindlimb reflex responses and coordinated motor function. Chronic lesion histopathology was evaluated by light microscopy and analyzed with morphometric techniques. All groups demonstrated profound functional deficits after injury followed by gradual recovery. Recovery correlated with the weight dropped and percent of white matter spared that was 41.3 +/- 6.0% (mean +/- SEM) in the 2 g x 2.5 cm group and 24.3 +/- 5.0% in the 3 g x 2.5 cm group. A replicate experiment confirmed reproducibility of the injury, This new mouse model of contusive SCI could pave the way for in vivo studies of the effect of genetic modifications produced by specific mutations on injury and recovery processes after spinal cord trauma.