3 ADDITIONAL GENES REQUIRED FOR DEOXYRIBONUCLEIC ACID SYNTHESIS IN SACCHAROMYCES-CEREVISIAE
3 ADDITIONAL GENES REQUIRED FOR DEOXYRIBONUCLEIC ACID SYNTHESIS IN SACCHAROMYCES-CEREVISIAE
复制标题
DOI：
10.1128/jb.115.3.966-974.1973
复制
发表时间：
1973-01-01
影响因子：
3.2
通讯作者：
HARTWELL, LH
中科院分区：
文献类型：
--
作者：
HARTWELL, LH
在一些cdc（细胞分裂周期）温度敏感突变株的异步和同步培养物中检测了脱氧核糖核酸（DNA）的合成。转移到限制性温度后的DNA合成动力学与在允许温度下抑制蛋白质合成后获得的动力学进行了比较，允许温度是一种特异性阻断新一轮DNA复制启动的条件，但不阻断正在进行的DNA复制。三个基因（cdc4、7和28）的突变似乎阻断了DNA合成的先决条件，因为携带这些病变的细胞在从允许温度转变为限制温度后不能开始新一轮的DNA复制，但可以完成正在进行的几轮复制。这三个基因被归类为在DNA合成的“起始”中起作用。两个基因（cdc8和cdc21）的突变本身就阻断了DNA合成，因为在允许温度下开始DNA合成的细胞携带这些病变，在转变到限制温度时突然停止合成。在限制性温度下，其他13个基因的突变不损害第一个细胞周期的DNA合成。
Deoxyribonucleic acid (DNA) synthesis was examined in asynchronous and synchronous cultures of a number ofcdc(cell division cycle) temperature-sensitive mutant strains. The kinetics of DNA synthesis after a shift to the restrictive temperature was compared with that obtained after inhibition of protein synthesis at the permissive temperature, a condition that specifically blocks the initiation of new rounds of DNA replication, but does not block those in progress. Mutations in three genes (cdc4, 7, and 28) appear to block a precondition for DNA synthesis since cells carrying these lesions cannot start new rounds of DNA replication after a shift from permissive to restrictive temperature, but can finish rounds that were in progress. These three genes are classified as having roles in the “initiation” of DNA synthesis. Mutations in two genes (cdc8 and 21) block DNA synthesis, itself, since cells harboring these lesions that had started DNA synthesis at the permissive temperature arrest synthesis abruptly upon a shift to the restrictive temperature. Mutations in 13 othercdcgenes do not impair DNA synthesis in the first cell cycle at the restrictive temperature.