A PLANT SIGNAL SEQUENCE ENHANCES THE SECRETION OF BACTERIAL CHIA IN TRANSGENIC TOBACCO
A PLANT SIGNAL SEQUENCE ENHANCES THE SECRETION OF BACTERIAL CHIA IN TRANSGENIC TOBACCO
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DOI：
10.1007/bf00018455
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发表时间：
1992-01-01
影响因子：
5.1
通讯作者：
DUNSMUIR, P
中科院分区：
文献类型：
--
作者：
LUND, P;DUNSMUIR, P
当分泌的细菌蛋白chia在转基因烟草中表达时，一部分蛋白被糖基化并从植物细胞中分泌出来，但大部分蛋白仍留在细胞内。我们测试了用植物信号序列取代细菌信号序列是否可以提高分泌效率。我们发现连接在CHIA成熟蛋白上的PR1b蛋白的信号序列和前两个氨基酸指导着植物细胞CHIA的完整糖基化和分泌。这种蛋白质的糖基化不是植物细胞高效分泌所必需的。
When the secreted bacterial protein ChiA is expressed in transgenic tobacco, a fraction of the protein is glycosylated and secreted from the plant cells; however most of the protein remains inside the cells. We tested whether the efficiency of secretion could be improved by replacing the bacterial signal sequence with a plant signal sequence. We found the signal sequence and the first two amino acids of the PR1b protein attached to the ChiA mature protein directs complete glycosylation and secretion of the ChiA from plant cells. Glycosylation of this protein is not required for its efficient secretion from plant cells.