Studying the interaction between trinuclear ruthenium complexes and human serum albumin by means of fluorescence quenching
Studying the interaction between trinuclear ruthenium complexes and human serum albumin by means of fluorescence quenching
复制标题
DOI：
10.1016/j.jlumin.2015.08.066
复制
发表时间：
2016-01-01
影响因子：
3.6
通讯作者：
Nikolaou, Sofia
中科院分区：
文献类型：
--
作者：
Cacita, Natacha;Nikolaou, Sofia
本工作首次报道了利用荧光光谱和Stern-Volmer模型系统地研究了HSA与三核钌配合物之间的相互作用。所研究的化合物具有通式[Ru_3 O（CH_3COO）（6）（3-pic）（2）（L）] PF_6，其中L=NO或H_2 O，3-pic=3-甲基吡啶。对于这两种复合物，观察到K-SV值随温度升高而增加（约10（4）M-1），表明HSA荧光的动态淬灭。然而，猝灭速率常数和K-b值的分析表明，静态猝灭的贡献是显着的。特别是在亚硝酰基复合物的情况下，观察到的相对高的K-b值（178.44 × 10（3）M-1，308 K）表明该化合物可以通过HSA在体内有效地储存和转运。复合物与HSA的相互作用是自发的（Δ G < 0）。配合物[Ru 3 O（CH 3COO）（6）（3-pic）（2）（L）] PF 6通过疏水作用与HSA相互作用（Δ H=215 kJ mol（-1）和Δ S=796 J mol（-1）K-1），可能是由于亚硝酰亲脂性，配合物Ru_3O（CH_3COO）（6）（3-pic）（2）（L）] PF_6与HSA形成氢键（Δ H= -75.5 kJ mol（-1）和Δ S = -231 J mol（-1）K-1）。(C)2015 Elsevier B. V.版权所有。
This work reports for the first time a systematic investigation of the interaction between HSA and trinuclear ruthenium complexes, by using fluorescence spectroscopy and the Stern-Volmer model. The compounds investigated have general formula [Ru3O(CH3COO)(6)(3-pic)(2)(L)]PF6, where L=NO or H2O and 3-pic=3-methylpyridine. For both complexes it was observed that the increase of K-SV, values (in the order of 10(4) M-1) with increasing temperature, signaling a dynamic quenching of HSA fluorescence. However, analysis of the quenching rate constants and K-b values shows that the contribution of the static quenching is significant. Particularly in the case of the nitrosyl complex, the relatively high value of K-b observed (178.44 x 10(3) M-1, 308 K) suggests that this compound can be efficiently stored and transported in the body by HSA. The interaction of the complexes with HSA is spontaneous (Delta G < 0). Complex [Ru3O(CH3COO)(6)(3-pic)(2)(L)]PF6 displays interaction with HSA by hydrophobic forces (Delta H=215 kJ mol(-1) and Delta S=796 J mol(-1) K-1), likely because of the nitrosyl lipophilicity, while complex Ru3O(CH3COO)(6)(3-pic)(2)(L)]PF6 is involved in the formation of hydrogen bonds with HSA (Delta H= -75.5 kJ mol(-1) and AS = -231 J mol(-1) K-1), through its aquo ligand. (C) 2015 Elsevier B.V. All rights reserved.