Dairy farm reservoir of Listeria monocytogenes sporadic and epidemic strains
Dairy farm reservoir of Listeria monocytogenes sporadic and epidemic strains
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DOI：
10.4315/0362-028x-67.11.2496
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发表时间：
2004-11-01
影响因子：
2
通讯作者：
Hu, JX
中科院分区：
文献类型：
--
作者：
Borucki, MK;Reynolds, J;Hu, JX
确定病原体的宿主对于控制散发疾病和流行病至关重要。单核细胞增生李斯特菌是一种人畜共患食源性病原体，每年在美国造成28%的食品相关死亡，也是全球大规模产品召回的主要原因。为了研究奶牛场作为引起人类李斯特菌病的单核增生乳杆菌亚型的潜在来源或储存库的作用，我们比较了单核增生乳杆菌奶牛场相关菌株（牛奶、环境和牛）与人类散发和流行疾病菌株的脉冲场凝胶电泳（PFGE）限制性内切酶酶切谱。23%的人类散发菌株具有与农场分离株相同的PFGE模式。此外，三种农场环境菌株和一种人类散发菌株具有与1985年加利福尼亚流行的单核细胞增生乳杆菌菌株相同的PFGE模式。这些数据表明，这一流行毒株继续引起散发的人类疾病，并有一个潜在的奶牛场作为水库。
Identifying the reservoirs of a pathogen is vital for control of sporadic disease and epidemics. Listeria monocytogenes is a zoonotic foodborne pathogen that is responsible for 28% of food-related deaths in the United States annually, as well as a major cause of massive product recalls worldwide. To examine the role of the dairy farm as a potential source or reservoir for L. monocytogenes subtypes shown to cause human listeriosis, we compared the pulsed-field gel electrophoresis (PFGE) restriction enzyme digestion profiles of L. monocytogenes dairy farm-associated strains (milk, environmental, and bovine) to human sporadic and epidemic disease strains. Twenty-three percent of human sporadic strains had PFGE patterns identical to that of farm isolate(s). Additionally, three farm environmental strains and one human sporadic strain had a PFGE pattern identical to a strain of L. monocytogenes responsible for the 1985 California epidemic. These data indicate that this epidemic strain continues to cause sporadic human illness and has a potential dairy farm as a reservoir.