Implantation of Completely Biological Engineered Grafts Following Decellularization into the Sheep Femoral Artery
Implantation of Completely Biological Engineered Grafts Following Decellularization into the Sheep Femoral Artery
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DOI：
10.1089/ten.tea.2013.0550
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发表时间：
2014-06-01
影响因子：
4.1
通讯作者：
Tranquillo, Robert T.
中科院分区：
文献类型：
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作者：
Syedain, Zeeshan H.;Meier, Lee A.;Tranquillo, Robert T.
在绵羊模型中评估了完全生物的、脱细胞的工程同种异体移植物的性能，以确定这些独特的同种异体动脉移植物的临床潜力。直径4 mm，长2-3 cm的移植物是由绵羊真皮成纤维细胞在体外将纤维蛋白凝胶改建成排列的组织管制成的。脱细胞和后续储存对移植物性能影响不大，破裂压力超过4000毫米汞柱，顺应性与羊股动脉相同。将移植物植入6只绵羊(n=9)股动脉，对侧假手术对照(n=3)。术后8周(n=5)和24周(n=4)，所有移植物均通畅，无扩张或矿化迹象。移植物中段管腔直径无变化。出现了广泛的再细胞化，大多数细胞表达α-SMA。24周时内皮化完全，弹性蛋白沉积明显。这些完全生物移植物具有天然动脉的周向排列/机械各向异性特征，在脱细胞和储存前仅培养5周，为“现成”移植物。
The performance of completely biological, decellularized engineered allografts in a sheep model was evaluated to establish clinical potential of these unique arterial allografts. The 4-mm-diameter, 2-3-cm-long grafts were fabricated from fibrin gel remodeled into an aligned tissue tube in vitro by ovine dermal fibroblasts. Decellularization and subsequent storage had little effect on graft properties, with burst pressure exceeding 4000mmHg and the same compliance as the ovine femoral artery. Grafts were implanted interpositionally in the femoral artery of six sheep (n = 9), with contralateral sham controls (n = 3). At 8 weeks (n = 5) and 24 weeks (n = 4), all grafts were patent and showed no evidence of dilatation or mineralization. Mid-graft lumen diameter was unchanged. Extensive recellularization occurred, with most cells expressing alpha SMA. Endothelialization was complete by 24 weeks with elastin deposition evident. These completely biological grafts possessed circumferential alignment/mechanical anisotropy characteristic of native arteries and were cultured only 5 weeks prior to decellularization and storage as "off-the-shelf'' grafts.