Expression analysis of calmodulin and calmodulin-like genes from rice, Oryza sativa L.
Expression analysis of calmodulin and calmodulin-like genes from rice, Oryza sativa L.
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DOI：
10.1186/1756-0500-5-625
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发表时间：
2012-11-08
影响因子：
1.8
通讯作者：
Buaboocha T
中科院分区：
文献类型：
--
作者：
Chinpongpanich A;Limruengroj K;Phean-O-Pas S;Limpaseni T;Buaboocha T
在植物中，钙调素（CaM）和类钙调素（CML）蛋白家族通过与靶蛋白结合并改变靶蛋白的活性来调节细胞内Ca ~（2+）浓度的增加，从而影响植物对各种刺激的生理反应。本文对水稻Cam和CML基因家族成员的转录表达进行了分析。进行了广泛的检查。水稻Cam和CML基因在组织器官中表现出差异表达。在渗透胁迫和盐胁迫条件下，OsCam 1 -1、OsCML 4、5、8和11的表达受到不同程度的诱导。OsCML 4和OsCML 8的mRNA表达在处理后3 h显著升高，并持续升高至少24 h，而OsCam 1 -1、OsCML 5和OsCML 11的表达在处理后1-3 h即开始上调，随后迅速恢复至正常水平。在这些基因的5′上游区域检测到了一些响应非生物胁迫的顺式作用元件，包括DREs（响应干旱、高盐和低温胁迫的重要启动子元件）。所观察到的通过OsCam 1 -1启动子的转基因水稻植株的GUS活性的诱导似乎是双相的，并且依赖于盐胁迫的严重程度。OsCam和OsCML基因大家族成员可能在植物的各种发育过程中发挥不同的信号转导作用，是植物非生物胁迫应答的信号转导和转录调控网络中的重要节点，这些非生物胁迫应答由复杂的Ca 2+信号介导，具有丰富的时空信息。
In plants, a large family of calmodulin (CaM) and CaM-like (CML) proteins transduce the increase in cytosolic Ca2+ concentrations by binding to and altering the activities of target proteins, and thereby affecting the physiological responses to a vast array of stimuli. Here, transcript expression analysis of Cam and CML gene family members in rice (Oryza sativa L.) was extensively examined. Cam and CML genes in rice exhibited differential expression patterns in tissues/organs. Under osmotic stress and salt stress, expression of OsCam1-1, OsCML4, 5, 8, and 11 was induced with different kinetics and magnitude. OsCML4 and 8 mRNA levels significantly increased by 3 h after treatment and remained elevated for at least 24 h while expression of OsCam1-1, OsCML5 and 11 was up-regulated as early as 1–3 h before rapidly returning to normal levels. Several cis-acting elements in response to abiotic stresses, including DREs (important promoter elements responsive to drought, high salt, and cold stress), were detected in the 5′ upstream regions of these genes. The observed induction of the GUS activity of transgenic rice plants via the OsCam1-1 promoter appeared to be biphasic and dependent on the severity of salt stress. Large OsCam and OsCML gene family members likely play differential roles as signal transducers in regulating various developmental processes and represent important nodes in the signal transduction and transcriptional regulation networks in abiotic stresss responses mediated by the complex Ca2+ signals in plants, which are rich in both spatial and temporal information.