Transcriptional regulation of receptor-like protein genes by environmental stresses and hormones and their overexpression activities in Arabidopsis thaliana.
Transcriptional regulation of receptor-like protein genes by environmental stresses and hormones and their overexpression activities in Arabidopsis thaliana.
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环境胁迫和激素对受体样蛋白基因的转录调控及其在拟南芥中的过表达活性
DOI：
10.1093/jxb/erw152
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发表时间：
2016-05
影响因子：
6.9
通讯作者：
Wang G
中科院分区：
文献类型：
--
作者：
Wu J;Liu Z;Zhang Z;Lv Y;Yang N;Zhang G;Wu M;Lv S;Pan L;Joosten MH;Wang G
转录分析表明，单个AtRLP基因可能参与多种生理过程。过表达研究发现AtRLP 3/11参与分生组织的发育，AtRLP 28参与耐盐性。受体样蛋白（Receptor-like Proteins，RLP）参与植物的生长发育和抗微生物感染等多种生物学过程。57 AtRLP基因已被确定在拟南芥中，而只有少数已被功能特性。这是由于缺乏合适的生理筛选条件和AtRLP基因之间的高度功能冗余。为了克服功能冗余和进一步了解AtRLP基因的作用，我们研究了AtRLP基因的进化，并编制了一个全面的档案AtRLP基因的转录调控后，暴露于一系列的环境压力和不同的激素。这些结果表明，大多数AtRLP基因的差异表达的各种条件下进行了测试，观察，这将有助于选择某些参与特定的生物过程的AtRLP基因进行进一步的实验研究，最终剖析其功能。大量的AtRLP基因被发现响应于一个以上的处理，这表明一个单一的AtRLP基因可能参与多个生理过程。此外，我们进行了全基因组克隆的AtRLP基因，并产生和表征过表达的个别AtRLP基因的转基因拟南芥植物，提出了新的见解AtRLP基因的作用，如AtRLP 3，AtRLP 11和AtRLP 28的例子。我们的研究概述了AtRLP基因可能参与的生物学过程，并为将来研究这些基因的功能提供了宝贵的资源。
Transcriptional analyses revealed that single AtRLP genes may be involved in multiple physiological processes. Overexpression studies found AtRLP3/11 to be involved in meristem development and AtRLP28 to be involved in salt tolerance. Receptor-like proteins (RLPs) have been implicated in multiple biological processes, including plant development and immunity to microbial infection. Fifty-seven AtRLP genes have been identified in Arabidopsis, whereas only a few have been functionally characterized. This is due to the lack of suitable physiological screening conditions and the high degree of functional redundancy among AtRLP genes. To overcome the functional redundancy and further understand the role of AtRLP genes, we studied the evolution of AtRLP genes and compiled a comprehensive profile of the transcriptional regulation of AtRLP genes upon exposure to a range of environmental stresses and different hormones. These results indicate that the majority of AtRLP genes are differentially expressed under various conditions that were tested, an observation that will help to select certain AtRLP genes involved in a specific biological process for further experimental studies to eventually dissect their function. A large number of AtRLP genes were found to respond to more than one treatment, suggesting that one single AtRLP gene may be involved in multiple physiological processes. In addition, we performed a genome-wide cloning of the AtRLP genes, and generated and characterized transgenic Arabidopsis plants overexpressing the individual AtRLP genes, presenting new insight into the roles of AtRLP genes, as exemplified by AtRLP3, AtRLP11 and AtRLP28. Our study provides an overview of biological processes in which AtRLP genes may be involved, and presents valuable resources for future investigations into the function of these genes.